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同种异体小鼠腹腔异位心脏移植模型是技术复杂的小动物显微手术。对于初学者必须经历一个学习曲线过程。学习曲线包括三个阶段:(1)熟悉供体和受体小鼠的局部解剖;(2)掌握供体小鼠心脏采集和受体小鼠腹腔大血管的准备;(3)掌握大血管的吻合技术。学习曲线的瓶颈在于大血管的吻合技术。学习曲线中的渐进要点是“认识、熟悉、准备、提速”。
Allogeneic mouse peritoneal heterotopic cardiac transplantation model is a technically complex micro-animal surgery. For beginners to go through a learning curve process. The learning curve consists of three stages: (1) being familiar with the local anatomy of donor and recipient mice; (2) mastering the preparation of donor mouse heart and collecting the large intraperitoneal blood vessels of recipient mice; (3) Anastomosis technology. The bottleneck of the learning curve lies in the anastomosis technique of large blood vessels. The gradual point in the learning curve is “know, be familiar with, prepare, speed up ”.