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Objective:To investigate the antioxidant,antibacterial and cytotoxic activity of whole Leucas aspera(Labiatae)(L.aspera)alcoholic extract.Methods:Whole L.aspera powder was extracted by absolute ethanol(99.50%).The ethanolic extract was subjected to antioxidant,antibacterial and brine shrimp lethality assay.Results:The extract showed potent radical scavenging effect(antioxidant)with IC_(50)value of(99.58±1.22)μg/mL which was significant(P<0.01)in comparison to ascorbic acid with IC_(50)value of(1.25±0.95)μg/mL.In case of antibacterial screening,the extract showed notable antibacterial effect against the tested microbial strains.Significant(P<0.05)zone of inhibitions against Gram positive Bacillus subtilis[(12.00±1.32)mm]and Bacillus megaterium[(13.00±1.50)mm],Staphylococcus aureus[(8.00±0.50)mm]and Gram negative Salmonella typhi[(6.00±0.50)mm],Salmonella paratyphi[(8.00±1.00)mm],Shigella dysenteriae[(9.00±1.32)mm]and Vibrio cholerae[(9.00±0.66)mm]was observed.In brine shrimp lethality bioassay,the extract showed the LC_(50)value as(181.68±2.15)μg/mL which was statistically significant(P<0.01)compared to positive control vincristine sulfate[LC_(50)=(0.76±0.04)μg/mL].Conclusions:The results demonstrate that the ethanolic extract of L.aspera could be used as antibacterial,pesticidal and various pharmacologic actives.
Objective: To investigate the antioxidant, antibacterial and cytotoxic activity of whole Leucas aspera (Labiatae) (L.aspera) alcoholic extract. Methods: Whole L. aspera powder was extracted by absolute ethanol (99.50%). , antibacterial and brine shrimp lethality assay. Results: The extract showed potent radical scavenging effect (antioxidant) with IC 50 value of (99.58 ± 1.22) μg / mL which was significant (comparison of ascorbic acid with IC_ (50) value of (1.25 ± 0.95) μg / mL.In case of antibacterial screening, the extract showed not notable antibacterial effect against the tested microbial strains .Significant (P <0.05) zone of inhibitions against Gram positive Bacillus subtilis [(12.00 ± Salmonella paratyphi [(8.00 ± 1.00) mm] and Gram negative Salmonella typhi [(6.00 ± 0.50) mm], Staphylococcus aureus [(8.00 ± 0.50) , Shigella dysenteriae [(9.00 ± 1.32) mm] and Vibrio cholerae [(9.00 ± 0.66) mm] was observed.In brine shrimp lethality bioassay, the extract showed the LC_ (50) value as (181.68 ± 2.15) μg / mL compared to the positive control vincristine sulfate [LC_ (50) = (0.76 ± 0.04) μg / mL ] .Conclusions: The results demonstrate that the ethanolic extract of L.aspera could be used as antibacterial, pesticidal and various pharmacologic actives.