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应用聚合酶链反应（ＰＣＲ）技术，扩增出完整的丙型肝炎病毒核心蛋白基因，将其克隆于表达载体ｐＢＶ２２０ＰＲＰＬ启动子的下游，构建了重组质粒ｐＢＶ－ＨＣＣ；转化大肠杆菌后，以非融合会自方式表达了丙型肝炎病毒核心蛋白。ＳＤＳ－ＰＡＧＥ电泳显示，此蛋白的分子量约为２０ｋＤａ，表达量约占菌体蛋白的１１％；Ｗｅｓｔｅｒｎ印迹实验证明，此蛋白能与慢性丙型肝炎患者血清发生特异反应。序列分析结果表明，克隆于ｐＢＶ２２０的外源基因确为丙型肝炎核心蛋白基因。以非融合蛋白形式表达的丙型肝炎核心蛋白，对深入研究丙型肝炎核心蛋白的结构、功能及生物学活性将起积极作用。
The complete hepatitis C virus core protein gene was amplified by polymerase chain reaction (PCR) and cloned into the downstream of the expression vector pBV220PRPL promoter to construct the recombinant plasmid pBV-HCC. After transformation of E. coli, Fusion will self-express the hepatitis C virus core protein. SDS-PAGE electrophoresis showed that the protein had a molecular weight of about 20 kDa and an expression level of about 11% of the bacterial protein. Western blotting showed that the protein reacted specifically with serum of patients with chronic hepatitis C virus. Sequence analysis showed that the foreign gene cloned in pBV220 was indeed the hepatitis C core protein gene. Hepatitis C core protein expressed as non-fusion protein will play an active role in further studying the structure, function and biological activity of hepatitis C core protein.