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目的研究CDC6、HSP27在宫颈癌组织中的表达及其与HPV16/18感染的相关性及其临床意义,探讨宫颈癌的发生机制。方法采用免疫组织化学方法检测50例宫颈癌、20例宫颈上皮内瘤变(cervical intraepithelial neopla-sia,CIN)CINⅠ、20例CINⅡ-Ⅲ、20例正常宫颈组织石蜡样品中CDC6、HSP27的表达,同时采用PCR技术检测HPV16/18感染情况。结果在宫颈癌组织中CDC6、HSP27的阳性率高于CIN和正常宫颈组织(均P<0.05),且CDC6、HSP27阳性表达率与病理分级和淋巴结转移有关(均P<0.05);HSP27阳性表达率与临床分期有关(P<0.05),CDC6阳性表达率与临床分期无关(P>0.05);在宫颈癌组织中CDC6和HSP27的阳性表达率与年龄分组无关(均P>0.05)。②HPV16/18在正常宫颈组织、CIN和宫颈癌中的阳性率逐渐升高(P<0.05),但与临床分期、病理分级、淋巴转移、年龄分组无关(均P>0.05)。③HSP27和CDC6、HPV16/18的表达无相关性(均P>0.05);CDC6与HPV16/18感染有关(P<0.05)。结论CIN及宫颈癌中CDC6表达的改变可能与HPV16/18感染有关,共同影响CIN的发展及宫颈癌的发生和发展。
Objective To study the expression of CDC6 and HSP27 in cervical cancer and their correlation with HPV16 / 18 infection and its clinical significance and to explore the mechanism of cervical cancer. Methods Immunohistochemistry was used to detect the expression of CDC6 and HSP27 in 50 cases of cervical cancer, 20 cases of cervical intraepithelial neoplasty (CIN) CINⅠ, 20 cases of CINⅡ-Ⅲ and 20 cases of normal cervical tissue. At the same time using PCR technology to detect HPV16 / 18 infection. Results The positive rates of CDC6 and HSP27 in cervical cancer tissues were higher than those in CIN and normal cervical tissues (all P <0.05). The positive rates of CDC6 and HSP27 were correlated with pathological grade and lymph node metastasis (all P <0.05) The positive rates of CDC6 and HSP27 in cervical cancer were not related with age (P> 0.05). The positive rates of CDC6 and HSP27 were not related with clinical stage (P> 0.05). ② The positive rate of HPV16 / 18 in normal cervical tissue, CIN and cervical cancer gradually increased (P <0.05), but not related to clinical stage, pathological grade, lymph node metastasis and age group (all P> 0.05). ③ There was no correlation between HSP27, CDC6 and HPV16 / 18 (all P> 0.05); CDC6 was associated with HPV16 / 18 infection (P <0.05). Conclusion The change of CDC6 expression in CIN and cervical cancer may be related to HPV16 / 18 infection, which may affect the development of CIN and the occurrence and development of cervical cancer.