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目的：通过观察影响ＨＣＶＣ基因在大肠杆菌中表达的因素，研制高表达工程菌株，制备重组抗原，用于免疫诊断和生物学功能研究。方法：将２种不同大小的ＨＣＶＣ基因片段克隆到带有ＰＬ启动子的表达载体中，通过ＳＤＳ－ＰＡＧＥ和免疫印迹分析基因表达产物。结果：在观察影响表达因素的基础上，获得了高表达工程菌株，其基因表达产物占菌体蛋白的３８．５％。结论：本研究表明，缺失疏水区段序列的ＨＣＶＣ基因片段在适宜的宿主细胞中可获得高效表达
OBJECTIVE: To observe the factors influencing the expression of HCVC gene in Escherichia coli (E.coli) and to develop high expression engineering strains to prepare recombinant antigens for immunodiagnosis and biological function research. METHODS: Two HCVC gene fragments of different sizes were cloned into an expression vector with a PL promoter and gene expression products were analyzed by SDS-PAGE and immunoblotting. Results: Based on the observation of the expression factors, the high expression engineering strain was obtained, whose gene expression product accounted for 38.5% of the bacterial protein. Conclusion: This study shows that the HCVC gene fragment lacking the hydrophobic segment sequence is highly expressed in a suitable host cell