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麻疹病毒融合蛋白的288-302位残基为小鼠辅助性T细胞表位,而404-414位残基为B细胞表位。作者将T细胞表位的氨基末端或者羧基末端通过两个甘氨酸臂分别与B细胞表位线性连接,合成两个嵌合肽TT/B和B/TT。以每剂2mg,加或不加霍乱毒素(CT)佐剂,经胃管口饲TO品系小鼠2～3次,间隔3周。采集接种小鼠血清,以合成肽包被的间接ELISA法测定其抗体滴度,并与~(125)I标记合成肽作液相放射免疫试验测定抗体对B细胞表位的亲和力,观察嵌合肽的免疫原性及小鼠的全身免疫应答。 结果显示,口服嵌合肽加CT的小鼠对两种嵌合肽和B细胞表位(404-414)肽均有抗体应答,且抗体特异性主要针对嵌合肽中的B表位部分;而口服嵌合肽加生理盐水的小鼠未产生抗体应答。
The 288-302 residues of the measles virus fusion protein are mouse helper T cell epitopes and the 404-414 residues are B cell epitopes. The authors synthesized two chimeric peptides, TT / B and B / TT, by linearly linking the amino-terminal or carboxy-terminal T-cell epitopes to B cell epitopes via two glycine arms, respectively. To each dose of 2mg, with or without cholera toxin (CT) adjuvant, TO mice were administered by mouth 2 to 3 times, 3 weeks apart. The sera of vaccinated mice were collected and the antibody titers were determined by indirect ELISA with synthetic peptide coating. The affinity of antibody to B cell epitopes was determined by liquid radioimmunity assay with ~ (125) I labeled synthetic peptide. Immunogenicity of peptides and systemic immune response in mice. The results showed that mice given oral chimeric peptide plus CT had an antibody response to both chimeric peptide and B cell epitope (404-414) peptide, and the antibody specificity was mainly directed against the B epitope in the chimeric peptide; Mice orally administered chimeric peptide plus normal saline did not produce an antibody response.