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目的 :观察缺血预处理对肝脏再灌注损伤的影响。方法 :通过构建正常大鼠和肝硬化大鼠肝脏 70 %的原位热缺血再灌注损伤模型 ,比较缺血预处理和无预处理组及间歇阻断肝门法对再灌注损伤的影响 ;以肝功酶、能量代谢和过氧化损伤等生物化学指标、组织学病理和细胞超微结构的形态学指标 ,观察不同预处理条件对结果的影响。结果 :各项指标显示 ,正常大鼠中 ,缺血预处理与无预处理组相比 ,可显著减轻再灌注损伤 (P <0 .0 5 ) ,与间歇性阻断肝门组相比也有显著性意义 (P <0 .0 5 ) ;在各个缺血预处理组内 ,缺血预处理 5min(IPC5min)组较IPC10min组和IPC5min× 2 组效果好 (P <0 .0 5 ) ,证实缺血预处理对肝硬化大鼠肝脏再灌注损伤有保护作用。结论 :缺血预处理可减轻肝脏再灌注损伤 ,并优于间歇性阻断肝门法 ,是一种应用方便、效果较好、前景广阔的阻断肝脏血供的新方法
Objective: To observe the effect of ischemic preconditioning on liver reperfusion injury. Methods: The model of 70% warm ischemia reperfusion injury was established in the liver of normal rats and cirrhotic rats. The effects of ischemic preconditioning and no preconditioning and intermittent hepatic portal approach on reperfusion injury were compared. The effects of different pretreatment conditions on the results were observed with the biochemical indicators such as liver enzyme, energy metabolism and peroxide damage, histopathology and ultrastructure of the cells. Results: All the indexes showed that compared with the non-preconditioning group, the ischemic preconditioning in normal rats could significantly reduce the reperfusion injury (P <0.05), compared with intermittent hepatic portal vein block (P <0.05). In each ischemic preconditioning group, ischemic preconditioning 5 min (IPC5 min) group was more effective than IPC10 min group and IPC5 min × 2 group (P <0.05), confirming Ischemic preconditioning has a protective effect on hepatic reperfusion injury in cirrhotic rats. Conclusion: Ischemic preconditioning can reduce hepatic reperfusion injury and is superior to intermittent hepatic portal approach, which is a new method that is convenient, effective and promising in blocking hepatic blood supply