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目的分析2010~2012年泰安市手足口病(Hand-foot-mouth Disease,HFMD)病例病原学检测结果,了解手足口病的流行病学和病原学特征,为防控手足口病提供科学依据。方法对2010~2012年泰安市临床诊断为手足口病的病例的1 474份粪便标本分别用RT-PCR、Realtime RT-PCR检测肠道病毒(PE)、CoxA16和EV71核酸,另结合流行病学资料进行分析。结果泰安市2010~2012年手足口病的发病高峰在4~8月,占全年发病总数的78.71%;全市6个县市区均有病例报告,尤其以宁阳、肥城发病率最高;发病人群以5岁以下儿童为高发人群,男性略多于女性。病原学检测粪便标本1 474份,其中,肠道病毒核酸阳性率为77.67%(1 145/1 474),CoxA16核酸阳性率为35.62%(525/1474),EV71核酸阳性率为24.49%(361/1 474),其他肠道病毒核酸阳性率为17.57%(259/1474)。结论 2010~2012年泰安市手足口病疫情以CoxA16、EV71感染引发病例为主,还存在其他肠道病毒感染引起的HFMD病例,采用分子生物学方法检测手足口病的病原体,对手足口病的诊断有重要作用。
Objective To analyze the etiological test results of hand-foot-mouth disease (HFMD) in Tai’an from 2010 to 2012 to understand the epidemiological and etiological characteristics of hand-foot-mouth disease and provide a scientific basis for the prevention and control of hand-foot-mouth disease. Methods A total of 1 474 stool specimens from clinical cases diagnosed as hand-foot-mouth disease in Tai’an from 2010 to 2012 were detected by Real-time RT-PCR and Real-time RT-PCR respectively. Data analysis. Results The peak incidence of HFMD in Tai’an from April to August, accounting for 78.71% of the total, was reported in all the districts in Tai’an City from 2010 to 2012. The incidence of HFMD in Ningxia and Feicheng was the highest The population of children under 5 years of age as a high incidence of the crowd, slightly more men than women. A total of 1 474 stool specimens were detected by etiological analysis. The positive rate of enterovirus nucleic acid was 77.67% (1 145/1 474), the positive rate of CoxA16 was 35.62% (525/1474), and the positive rate of EV71 was 24.49% (361) / 1 474). The positive rate of other enterovirus nucleic acids was 17.57% (259/1474). Conclusion From 2010 to 2012, the epidemic situation of HFMD in Tai’an City was mainly caused by CoxA16 and EV71 infection, and HFMD cases caused by other enterovirus infections were also detected. Pathogens of hand-foot-mouth disease were detected by molecular biology method, Diagnosis has an important role.