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近年来，对人和实验性肝纤维化发病机理的研究焦点主要集中于贮脂细胞（ＦＳＣ）的病理学改变，而有关正常或不完全损伤肝实质细胞（ＰＣ）在ＦＳＣ激活过程中的作用少见报道。本文对正常和ＣＣｌ４损伤大鼠ＰＣ进行分离培养，观察ＰＣ和库普弗细胞（ＫＣ）在ＦＳＣ激活过程中的协同作用。采用ＰＣ、ＫＣ和ＦＳＣ分离培养，用流式细胞仪测定ＤＮＡ含量，免疫组化和图像分析对ＦＳＣ中Ⅱ、Ⅲ、Ⅳ型胶原和ＦＮ进行定量分析，液闪法检测ＦＳＣ中３Ｈ－脯氨酸掺入量。来自正常或ＣＣｌ４损伤大鼠ＰＣ条件培养基（ＰＣｎｃｍ、ＰＣｔｃｍ）对原代培养ＦＳＣ的增殖具有较强的刺激效应，ＦＳＣ内３Ｈ－脯氨酸掺入量呈现时间和剂量相关性地升高，ＤＮＡ含量在时间和剂量上也呈正相关的增加。单独用ＰＣｎｃｍ或ＰＣｎｃｍ＋ＰＣｔｃｍ合用时，ＦＳＣ表现出明显地转化特征，而ＦＳＣ内Ⅰ、Ⅲ、Ⅳ型胶原和纤维粘连蛋白（ＦＮ）均显著升高。ＰＣ和ＫＣ对ＦＳＣ的激活具有协同效应，并呈现时空相关性，对ＦＳＣ内ＥＣＭ合成的协同效应与增加细胞总量及增加单个ＦＳＣ合成ＥＣＭ的能力有关。
In recent years, the focus of research on the pathogenesis of human and experimental liver fibrosis has focused primarily on the pathological changes in fat storage cells (FSC), and the role of normal or incomplete damage to the activation of hepatic parenchymal cells (PC) during FSC activation Rarely reported. In this paper, normal and CCl4 injured rat PC were isolated and cultured to observe the synergistic effect of PC and Kupffer cells (KC) during FSC activation. The PCC, KC and FSC were isolated and cultured. The DNA content was determined by flow cytometry. Immunohistochemistry and image analysis were used to quantitatively analyze the type Ⅱ, Ⅲ, Ⅳ collagen and FN in FSC. The level of 3H- Acid incorporation. The PC conditioned medium (PCncm, PCtcm) from normal or CCl4-injured rats had a strong stimulating effect on the proliferation of primary culture FSC. The incorporation of 3H-proline in FSC increased in time and dose dependent manner, DNA content also showed a positive correlation between time and dose. When combined with PCncm or PCncm + PCtcm alone, FSC showed obvious transformation characteristics, while the type Ⅰ, Ⅲ, Ⅳ collagen and fibronectin (FN) in FSC were significantly increased. PC and KC have a synergistic effect on the activation of FSC and exhibit spatiotemporal correlation, and the synergistic effect on ECM synthesis within the FSC is related to increasing cell mass and increasing the ability of individual FSCs to synthesize ECMs.