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目的:研究慢性肺源性心脏病患者血清中白细胞介素-9(IL-9)、白细胞介素-10(IL-10)、转录因子PU.1水平的变化,探讨在肺心病发病中的作用。方法:采用荧光定量逆转录聚合酶链反应法分别检测慢性肺心病患者60例,健康对照者30例血清中IL-9、IL-10及PU.1mRNA表达水平。结果:肺心病患者血清IL-9水平高于对照组(P<0.05)。肺心病患者血清IL-10显著低于对照组(P<0.01)。肺心病患者血清PU.1水平高于对照组(P<0.05)。IL-9与IL-10表达呈负相关(r=-0.745 P<0.05)。IL-10表达水平可能与疾病严重程度有关。结论:IL-9、IL-10、PU.1参与到疾病的发病过程,联合检测这三种因子对于慢性肺心病发病机制的探索及临床诊治具有重要的意义。
Objective: To study the changes of serum interleukin-9 (IL-9), interleukin-10 (IL-10) and transcription factor PU.1 in patients with chronic cor pulmonale, effect. Methods: The levels of IL-9, IL-10 and PU.1 mRNA in serum of 60 patients with chronic cor pulmonale and 30 healthy controls were detected by fluorescence quantitative reverse transcription polymerase chain reaction. Results: The level of serum IL-9 in patients with cor pulmonale was higher than that in the control group (P <0.05). Serum IL-10 in patients with cor pulmonale was significantly lower than that in the control group (P <0.01). Pulmonary heart disease patients with serum PU.1 levels higher than the control group (P <0.05). IL-9 and IL-10 expression was negatively correlated (r = -0.745 P <0.05). IL-10 expression levels may be related to the severity of the disease. CONCLUSION: IL-9, IL-10 and PU.1 are involved in the pathogenesis of the disease. The joint detection of these three factors is of great significance for the exploration of the pathogenesis of chronic cor pulmonale and clinical diagnosis and treatment.