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目的探讨化疗对噬菌体展示肽法检测大肠癌自身抗体效果的影响。方法选取前期研究筛选出的5个(95号,149号,174号,396号,1009号)能高效区分大肠癌患者血清和健康对照者血清的大肠癌相关噬菌体克隆作为检测噬菌体,采用酶联免疫吸附试验(ELISA)检测该5个检测噬菌体克隆与20例化疗大肠癌患者、40例非化疗大肠癌患者及40例健康对照者血清的反应性。结果 95号检测噬菌体与大肠癌化疗患者血清反应性低于与非化疗大肠癌患者血清反应性(P<0.05),该噬菌体在健康对照组及化疗大肠癌患者组的血清反应性差异无统计学意义(P=0.074)。149号、174号、396号、1009号噬菌体在健康对照组及化疗大肠癌患者组的血清反应性差异有统计学意义(P均<0.01)。结论化疗对149号、174号、396号、1009号噬菌体的检测效果影响不明显,而95号噬菌体对肿瘤自身抗体的检测能力受化疗影响明显。
Objective To investigate the effect of chemotherapy on the detection of autoantibodies to colorectal cancer by phage display peptide method. Methods Five colorectal cancer-associated phage clones (95, 149, 174, 396, 1009) screened from previous studies were used to distinguish colorectal cancer serum from healthy controls. Immunoabsorbent assay (ELISA) was used to detect the reactivity of the 5 detected phage clones with 20 patients with chemotherapy colorectal cancer, 40 patients with non-chemotherapy colorectal cancer and 40 healthy controls. Results Serum reactivity of No. 95 phage and colorectal cancer chemotherapy patients was lower than that of non-chemotherapy colorectal cancer patients (P <0.05). There was no significant difference in the serum reactivity of the phage between the control group and chemotherapy colorectal cancer patients Significance (P = 0.074). Serum reactivity of phage 149, 174, 396 and 1009 in the control group and chemotherapy group were significantly different (all P <0.01). Conclusion Chemotherapy has no obvious effect on the detection of phage 149, 174, 396 and 1009, while the detection of phage antibody 95 on tumor autoantibodies is obviously affected by chemotherapy.