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背景:目前治疗膝骨关节炎的药物主要是非类固醇类药,应用后可产生胃肠道副作用。研究表明,补肾益气活血方能明显改善膝骨关节炎患者关节疼痛和功能,并且副作用较小。目的:通过建立兔膝关节骨关节炎模型,探讨补肾益气活血方对兔病变膝关节软骨病理过程的影响。设计:以实验动物为研究对象,随机对照观察研究。单位:一所大学动物实验中心。材料:健康雄性新西兰兔36只,体质量2.0～3.0kg。方法:实验于2002-01/2003-01在广州中医药大学动物实验中心完成。新西兰兔36只,建立Hulth膝骨关节炎模型,术后随机分成3组:模型组、醋氨酚组和补肾益气活血方组。各组采取相应的处理方法,术后4,8和12周取材,进行组织病理学评估。主要观察指标:主要结局:各组关节软骨病理改变积分。次要结局:①各组关节软骨肉眼观察。②各组关节软骨光镜观察。结果:模型组、醋氨酚组、补肾益气活血方组关节软骨病理变化总积4周分别为(18.50±1.00),(14.25±1.26),(11.75±2.22)分;12周分别为(30.75±1.26),(22.00±3.16),(17.75±2.21)分。各组关节软骨病理变化总积分、软骨细胞病理改变积分和软骨表层病理改变积分依次为模型组>醋氨酚组>补肾益气活血方组。组间比较差异均有显著或非常显著性意义。结论:补肾益气活血方能延缓膝骨关节炎的病?
BACKGROUND: Currently, the drugs for treating knee osteoarthritis are mainly non-steroidal drugs, which can produce gastrointestinal side effects after application. Studies have shown that Bushen Yiqi Huoxue can significantly improve joint pain and function in patients with knee osteoarthritis, and have fewer side effects. OBJECTIVE: To investigate the effect of Bushen Yiqi Huoxue Recipe on the pathological process of knee joint cartilage in rabbits by establishing a knee osteoarthritis model in rabbits. Design: Randomized controlled observational study of experimental animals. Unit: A university animal experiment center. MATERIALS: Thirty-six healthy male New Zealand rabbits weighing 2.0-3.0 kg. METHODS: The experiment was performed at the Animal Experimental Center of Guangzhou University of Traditional Chinese Medicine from January 2002 to January 2003. Thirty-six New Zealand rabbits were used to establish the Hulth knee osteoarthritis model and randomly divided into three groups: model group, acetaminophen group, and Bushen Yiqi Huoxue Recipe group. Each group took corresponding treatment methods, and obtained materials at 4, 8, and 12 weeks after operation for histopathological evaluation. MAIN OUTCOME MEASURES: Main Outcomes: Pathological changes in joint cartilage in each group. Secondary outcome: 1 The articular cartilage of each group was visually observed. 2 Light microscope observation of articular cartilage in each group. Results: The total pathological changes of articular cartilage in model group, acetaminophen group and Bushen Yiqi Huoxue Recipe group were (18.50±1.00), (14.25±1.26), (11.75±2.22) points for 4 weeks, respectively; 30.75±1.26), (22.00±3.16), (17.75±2.21) points. The total pathological changes of joint cartilage, the pathological changes of chondrocytes and the pathological changes of cartilage surface in each group were model group> acetaminophen group> Bushenyiqihuoxue prescription group. There was significant or very significant difference between groups. Conclusion: Bushen Yiqi Huoxue can delay the disease of knee osteoarthritis?