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目的：研究神经肽 Ｙ 对血管平滑肌细胞增殖的影响。方法：应用快速自动微量 Ｍ Ｔ Ｔ比色法和荧光免疫组化定量技术观察缩血管物质神经肽 Ｙ 对体外培养血管平滑肌细胞的 Ｍ Ｔ Ｔ比色吸光度和细胞核增殖抗原（ Ｐ Ｃ Ｎ Ａ） 表达的平均荧光值。结果：神经肽 Ｙ 作用下血管平滑肌细胞的增殖活度和 Ｐ Ｃ Ｎ Ａ 表达比对照组明显升高。 Ｍ Ｔ Ｔ值在 Ｎ Ｐ Ｙ 组和对照组中分别为０２６２６ ±００ ０２５ 和０２ ２３９ ±００ ０１０ ，两者差异明显（ Ｐ ＜ ００１） ；而 Ｐ Ｃ Ｎ Ａ 表达的平均荧光值， Ｎ Ｐ Ｙ 组为１ ６４８５６ ±２３２７１ ，对照组为１５４２７１ ±２０００４ ，两者有差异（ Ｐ ＜ ００５） 。结论：神经肽 Ｙ 可影响血管平滑肌细胞的增殖活动；血中神经肽 Ｙ 浓度的变化可能是高血压时血管壁结构变化的重要因素之一。
Objective: To study the effect of neuropeptide Y on the proliferation of vascular smooth muscle cells. Methods: Expressions of neurotrophin Y, a vasoconstrictor substance, on M T T colorimetric absorbance and P C N A expression in cultured vascular smooth muscle cells (VSMCs) in vitro were observed by rapid and automatic microtitration MTT assay and fluorescence immunohistochemistry The average fluorescence value. Results: Proliferation activity and P C N A expression of vascular smooth muscle cells were significantly increased compared with the control group. The values of M T T were 0.2626 ± 0025 and 02 239 ± 00 010 in the NPY group and the control group, respectively (P <001) The average fluorescence value of A was 1664 ± 23271 in N P Y group and 154271 ± 20004 in control group (P <005). CONCLUSION: Neuropeptide Y can affect the proliferation of vascular smooth muscle cells. The change of neuropeptide Y concentration in blood may be one of the important factors in the changes of vascular wall structure in patients with hypertension.