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目的预测人乳头瘤病毒16型L1(HPV-16 L1)蛋白的B细胞表位。方法先从NCBI的蛋白质数据库中获得HPV-16 L1蛋白的氨基酸序列,再采用DNAStar中的Protean模块分析HPV-16型L1蛋白的二级结构、柔韧性、亲水性、表面可及性以及抗原性,然后结合吴玉章等建立的氨基酸抗原性指数方法计算其平均抗原指数(AI),综合分析上述多个参数,预测其可能的B细胞表位区域,并用在线BLAST进行同源性匹配分析。结果综合分析多个参数,预测得出HPV-16 L1蛋白的B细胞表位可能位于第51~58、87~97、214~220、290~296、335~341、351~366、408~418、430~442和475~496肽段,进一步的同源性匹配分析显示肽段51~58、335~341、351~366、408~418、430~442以及475~496为其B细胞表位优势区域,其中肽段51(PIKKPNNN)58、351(SETTYKNTNFKEYLRH)366、408(PPPGGTLEDTY)418和430(KHTPPAPKEDPLK)442的氨基酸序列为HPV-16型L1蛋白所特有,而肽段475(KAKPKFTLGKRKATPTTSSTST)496与HPV-16 E1蛋白具有同源性、335(DTTRSTN)341的氨基酸序列与很多其他型别的HPV的L1蛋白具有同源性。结论综合多种方案预测得到HPV-16 L1的多个B细胞表位优势区域。
Objective To predict the B cell epitope of human papillomavirus type 16 L1 (HPV-16 L1) protein. Methods The amino acid sequence of HPV-16 L1 protein was obtained from the protein database of NCBI. The secondary structure of HPV-16 L1 protein was analyzed by ProteStar module in DNAStar. The flexibility, hydrophilicity, surface accessibility and antigen Then, the average antigen index (AI) was calculated by amino acid antigen index established by Wu Yuzhang et al. The above parameters were comprehensively analyzed to predict the possible B cell epitope regions. Homology matching analysis was performed by online BLAST. Results A comprehensive analysis of multiple parameters predicted that the B cell epitopes of HPV-16 L1 protein might be located in the region of 51-57,77,214-220,290-296,335-3 341,351-366,408-418 , 430 ~ 442 and 475 ~ 496 peptides, further homology analysis showed that peptides 51 ~ 58,335 ~ 341,351 ~ 366,408 ~ 418,430 ~ 442 and 475 ~ 496 were their B cell epitopes The amino acid sequence of the peptide region 51 (PIKKPNNN) 58,351 (SETGNTNFKEYLRH) 366,408 (PPPGGTLEDTY) 418 and 430 (KHTPPAPKEDPLK) 442 is peculiar to HPV-16 type L1 protein while peptide 475 (KAKPKFTLGKRKATPTTSSTST) 496 Has homology to the HPV-16 El protein, and 335 (DTTRSTN) 341 has homology to the L1 protein of many other types of HPV. Conclusions Multiple B-cell epitope predominant regions of HPV-16 L1 were predicted by comprehensive methods.