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本文通过小鼠腹腔内移注高度激活的巨噬细胞,显示腹腔炎症对早期生殖过程的抑制作用,以及相应免疫调节药物对上述抑制的消除作用。从而提出了治疗轻度子宫内膜异位不孕的新方法以替代当前流行的激素或手术疗法。在小鼠腹腔内注射4%无菌硫羟乙酸盐培养液4ml,72小时后处死收集腹腔冲洗液,经离心、孵育,制备成激活的巨噬细胞。未激活巨噬细胞则从直接收集不注射此药小鼠的腹腔液获得。另取61只6周龄同系雌鼠作为实验对象。先以孕马血清促性腺激素使卵巢过度刺激,然后分6组腹腔内分别注射以下物质:①培养液,即对照组(n=12)。②培养液并皮下注射己酮可可碱(n=10)。③未激活巨噬细胞(n=10)。④激活巨噬细胞(n=12)。
In this paper, intraperitoneal transplants of highly activated macrophages in mice, the inhibition of early reproductive inflammation caused by peritoneal inflammation, and the corresponding immunomodulatory drugs to eliminate the above inhibition. Thus proposed a new method of treatment of mild endometriosis infertility to replace the current popular hormone or surgical therapy. 4 ml of 4% sterile thiolacetate solution was injected intraperitoneally into mice and 72 hours later, the peritoneal irrigating solution was collected, centrifuged and incubated to prepare activated macrophages. Inactive macrophages were obtained from peritoneal fluid collected directly from mice not injected with the drug. Another 61 6-week-old female mice were used as experimental subjects. First, pregnant mare serum gonadotropin ovarian hyperstimulation, and then divided into 6 groups intraperitoneal injection of the following substances: ① culture medium, the control group (n = 12). ② culture medium and subcutaneous injection of pentoxifylline (n = 10). ③ did not activate macrophages (n = 10). ④ activate macrophages (n = 12).