论文部分内容阅读
目的:建立大鼠“血瘀证”下急性心肌梗死模型,探讨其与单纯急性心肌梗死模型在血液生化学方面的差异,为评价治疗胸痹心痛中药的药效学提供理论依据。方法:Wistar大鼠随机分为空白对照组、急性血瘀模型组、急性心肌梗死假手术组、单纯急性心肌梗死模型组及“血瘀证”下急性心肌梗死模型组(每组12只),在大鼠急性“血瘀”情况下复制急性心肌梗死模型,测定各组大鼠血清丙二醛(MDA)、一氧化氮(NO)、游离脂肪酸(FFA)及肿瘤坏死因子α(TNF-α)含量,血清超氧化物歧化酶(SOD)及谷胱甘肽过氧化物酶(GSH-Px)活性,血浆前列环素(PGI2)、血栓素A2(TXA2)及内皮素(ET)水平,并与单纯急性心肌梗死模型大鼠进行比较。结果:“血瘀证”下急性心肌梗死模型大鼠血清MDA、SOD、GSH-Px及FFA水平与单纯急性心肌梗死模型大鼠比较差异无显著性(P>0.05),PGI2及NO的减少程度和TXA2、ET及TNF-α的增加程度均明显大于单纯急性心肌梗死模型组(P<0.05),TNF-α活性明显高于单纯急性心肌梗死模型组(P<0.05)。结论:大鼠“血瘀证”下急性心肌梗死模型与单纯急性心肌梗死模型在自由基对心肌的损伤及心肌缺血时FFA的代谢紊乱方面无明显区别,但在内源性血管活性物质的释放方面存在明显区别。提示在评价治疗胸痹心痛中药的药效学时,仅采用单纯的急性心肌梗死模型,不考虑中医的证候是有缺陷的。
OBJECTIVE: To establish a rat model of acute myocardial infarction under “blood stasis syndrome” and to explore the differences in blood biochemistry between the model and the simple acute myocardial infarction model in order to provide a theoretical basis for evaluating the pharmacodynamics of traditional Chinese medicine for the treatment of chest pain. METHODS: Wistar rats were randomly divided into blank control group, acute blood stasis model group, acute myocardial infarction sham operation group, simple acute myocardial infarction model group and “blood stasis syndrome” acute myocardial infarction model group (12 rats in each group). )In the rat acute “blood bleed” case of acute myocardial infarction model, determination of serum MDA, nitric oxide (NO), free fatty acid (FFA) and tumor necrosis factor α (TNF-α) content, serum superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) activity, plasma prostacyclin (PGI2), thromboxane A2 (TXA2) and endothelin ( ET) levels were compared with simple acute myocardial infarction model rats. RESULTS: There were no significant differences in serum MDA, SOD, GSH-Px and FFA levels between rats with acute myocardial infarction and blood stasis syndrome (P>0.05). The degree of reduction and the increase of TXA2, ET and TNF-α were significantly greater than those in the simple acute myocardial infarction model group (P<0.05), and the TNF-α activity was significantly higher than that in the simple acute myocardial infarction model group (P<0.05). Conclusion: There is no significant difference in the myocardial injury induced by free radicals and the metabolic disorder of FFA during myocardial ischemia in the rat model of acute myocardial infarction under the “blood stasis syndrome” and the simple acute myocardial infarction model, but the endogenous vascular activity There are clear differences in the release of substances. Tip When evaluating the pharmacodynamics of traditional Chinese medicine for the treatment of chest pain, only the simple acute myocardial infarction model is used, and the syndrome of Chinese medicine is not considered defective.