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目的了解山西省南部地区虫媒病毒的种类及分布特征。方法使用诱蚊灯捕蚊,通过组织培养法分离病毒,并对病毒分离物进行血清学和分子生物学鉴定。结果2009年9月在山西省运城市、临汾市和阳泉市的3个标本采集点采集到3属4种共3436只蚊虫标本,从中分离到5株病毒分离物,鉴定结果显示有3株(SX0621、SX0633、SX0635)为淡色库蚊浓核病毒,其余2株分离物有待进一步鉴定。对CppDNV非结构蛋白NS1和NS2的部分核苷酸序列分析显示,3株山西省新分离株的同源性为100%;与中国其他省市的分离株同源性在99.9%～100.0%之间。系统进化分析提示所有中国分离株均位于一个相对独立的进化分支中,并且与AaeDNV的进化关系较近。结论在山西省首次分离到淡色库蚊浓核病毒,与中国其他地区的分离株同源性较高。
Objective To understand the species and distribution of arboviruses in southern Shanxi Province. Methods The mosquito lamp was used to catch mosquitoes and the virus was isolated by tissue culture method. The virus isolates were identified by serology and molecular biology. Results In September 2009, 3436 mosquitoes from 3 genera and 4 species were collected from 3 specimens collected in Yuncheng City, Linfen City and Yangquan City of Shanxi Province. Five virus isolates were isolated from them. The identification results showed that there were 3 SX0621, SX0633, SX0635) were darkpot-bellows concentrated nucleocapsids, and the remaining 2 isolates were to be further identified. Partial nucleotide sequence analysis of CppDNV NS1 and NS2 showed that the homology of the three new isolates in Shanxi Province was 100%. The homology of isolates from other provinces in China was between 99.9% and 100.0% between. Phylogenetic analysis suggested that all Chinese isolates were located in a relatively independent evolutionary branch and had a close evolutionary relationship with AaeDNV. Conclusion The results showed that the first isolate of D. plicadense in Shanxi Province has high homology with other isolates in China.