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目的:探讨CD3AK细胞与LAK细胞的诱导、增殖动力学和杀伤活性的变化。方法:采用抗CD3单克隆抗体(anti-CD3McAb)和rIL-2及PHA(植物血凝素)共刺激法诱生扩增CD3AK细胞,用等量rIL-2和PHA共刺激法诱生LAK细胞,观察它们的增殖动力学变化;用MTT法以K562细胞和H7402细胞为靶细胞,测定CD3AK细胞和LAK细胞的杀伤活性。结果:微量的anti-CD3McAb辅以少量的rIL-2和PHA就能诱导和大量扩增CD3AK细胞,其扩增能力显著高于LAK细胞(P<0.001);CD3AK细胞对K562细胞和H7402细胞的杀伤活性显著高于LAK细胞(P<0.05)。结论:CD3AK细胞的扩增能力及杀伤活性均较LAK细胞为强,是更为有效的杀瘤效应细胞
Objective: To investigate the changes of the induction, proliferation kinetics and cytotoxicity of CD3AK cells and LAK cells. Methods: CD3AK cells were induced by anti-CD3 monoclonal antibody (anti-CD3McAb), costimulatory rIL-2 and PHA (phytohemagglutinin), and LAK cells were induced by the same amount of rIL-2 and PHA costimulation , And their proliferation dynamics were observed. K562 cells and H7402 cells were used as target cells by MTT assay to determine the killing activity of CD3AK cells and LAK cells. Results: A small amount of anti-CD3 McAb supplemented with a small amount of rIL-2 and PHA could induce large-scale expansion of CD3AK cells and its amplification ability was significantly higher than that of LAK cells (P <0.001); CD3AK cells had no effect on K562 cells and H7402 cells The killing activity was significantly higher than that of LAK cells (P <0.05). Conclusion: The ability of CD3AK cells to proliferate and kill is stronger than that of LAK cells, which is more effective to kill tumor-bearing effector cells