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鉴于血小板活化因子(PAF)是强力的血管活性物质,子宫血管通透性增加是胚囊植入的最早指征之一,本文研究了早孕和假孕期家兔子宫内PAF的浓度变化,以探讨PAF在与植入发生有关的各种过程中的潜在作用。实验选用体重3.0kg成年新西兰雌兔,于交配前连续3天每天皮下注射0.5U猪卵泡刺激素(FSH)二次,第4天将经人工阴道从雄兔收集到的混合精液0.5ml立即置入阴道内使家兔授精,授精后静脉注射1001U人绒毛膜促性腺激素(hCG)。假孕动物同样给与FSH和hCG,但不授精,动情期动物不给与任何处理。授精后不同时间处死动物,在4℃环境中迅速取出子宫角、卵巢、胚囊等,按修改的Folch等(1957)和Bligh等(1959)
In view of the fact that platelet activating factor (PAF) is a potent vasoactive substance, the increase of uterine vascular permeability is one of the earliest indications of embryo implantation. In this paper, the changes of PAF in rabbits in early pregnancy and pseudopregnancy were studied in order to investigate whether PAF Potential role in various processes related to implantation. In the experiment, adult New Zealand female rabbits weighing 3.0kg were injected subcutaneously with 0.5U follicle stimulating hormone (FSH) subcutaneously twice daily for 3 consecutive days prior to mating. On the 4th day, 0.5ml mixed semen collected from male rabbits by artificial vaginal immediately Into the vagina so that the rabbit insemination, insemination after intravenous injection of 1001U human chorionic gonadotropin (hCG). Fake pregnant animals are also given FSH and hCG, but not insemination, estrus animals do not give any treatment. Animals were sacrificed at different times after insemination and the uterine horns, ovaries, embryo sacs and the like were removed rapidly at 4 ° C. According to modified Folch et al. (1957) and Bligh et al. (1959)