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AIM: To investigate the inhibitory effect of tea polyphenols (TP)on the transforming growth factor-pi (TGF-β1) expression in rat model of cyclosporine A (CsA)-induced chronic nephrotoxicity. METHODS: The rat model of CsA-induced chronic nephrotoxicity was used, 4 groups of rats were respectively treated with vehicle (0.1 mL·lg-1-d-1 sc), TP (80 mg·kg-1·d-1 ig), CsA (15 mg·2kg-1·d-1sc) and TP plus CsA (CsA 15 mg·kg-1·d-1 sc+TP 80 mg·kg-1·d-1 ig). At the end of day 28 of treatment, serum and urine are analyzed for creatinine clearance, kidney tissue for pathologic analysis. The TGF-β1 mRNA and its protein expression were detected by RT-PCR, immunohistochemistry, and Western blot. RESULTS: CsA-treated rats had increased renal expression of TGF-pl mRNA and its protein, compared with the vehicle- or TP- treated
AIM: To investigate the inhibitory effect of tea polyphenols (TP) on the transforming growth factor-pi (TGF-β1) expression in rat model of cyclosporine A (CsA)-induced chronic nephrotoxicity. METHODS: The rat model of CsA-induced chronic Nephrotoxicity was used, 4 groups of rats was assigned treated with vehicle (0.1 mL·lg-1-d-1 sc), TP (80 mg·kg-1·d-1 ig), CsA (15 mg·2kg-1) D-1sc) and TP plus CsA (CsA 15 mg·kg-1·d-1 sc+TP 80 mg·kg-1·d-1 ig). At the end of day 28 of treatment, serum and urine are The analysis of creatinine clearance, kidney tissue for pathologic analysis. The TGF-β1 mRNA and its protein expression were detected by RT-PCR, immunohistochemistry, and Western blot. RESULTS: CsA-treated rats had increased renal expression of TGF-pl mRNA and its Protein, compared with the vehicle- or TP-treated