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用人睾丸制备长形精子细胞核及附睾制备附睾精子核，盐酸胍法提取总碱性蛋白，通过SephadexG－100分子筛层析，收集第3峰，经5％TCA沉淀后得到纯化的人精核蛋白（humanprotamine）．
Preparation of sperm nucleus and epididymis of human epididymis by human testis, total alkaline protein extracted by guanidine hydrochloride method, and the third peak was collected by SephadexG-100 molecular sieve chromatography. The purified human nucleoprotein humanprotamine).