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目的 研究六君子汤含药血清对人肺腺癌顺铂耐药细胞株(A549/DDP)顺铂(DDP)耐药的作用及其对上皮钙黏蛋白(E-cadherin)和神经钙黏蛋白(N-cadherin)表达的影响.方法 采用血清药理学方法制备含药血清,随机将40只SD大鼠分为血清对照组和六君子汤(高、中、低剂量)含药血清组,每日灌胃给药1次,连续5天后提取血清.再用培养液将各组血清稀释成5％和10％浓度作用于A549/DDP细胞株12h、24h、48h,以此筛选出最佳浓度及最佳作用时间点.MTT法检测各组细胞对DDP的IC50,计算耐药逆转倍数;免疫细胞化学法和Western blot检测各组细胞中E-eadherin和N-cadherin蛋白的表达.结果 六君子汤含药血清对A549/DDP细胞有明显抑制作用,且呈浓度和时间依赖关系,中剂量10％浓度含药血清作用48h作用较强且细胞毒性低,在此浓度下,TGF-β1诱导的A549/DDP的IC50明显下降(R0.05),对A549/DDP细胞的耐药逆转倍数为4.12,E-cadherin表达水平上调,N-cadherin的表达水平下调.结论 六君子汤含药血清逆转A549/DDP细胞的顺铂耐药可能与抑制细胞上皮-间质转化,增强A549/DDP对DDP的敏感性有关.“,”Objective To study the effect of serum containing Liu Junzi decoction on cisplatin resistance human adenocarcinoma of lung cell line A549/DDP,and the expression of E-cadherin and N-cadherin in A549/DDP.Methods The serum containing Liu Junzi decoction was prepared by serum pharmacology method,40 SD rats were divided into serum control group and serum containing Liu Junzi decoction (high,middle and low dose) groups.Liu Junzi decoction was orally given once a day for five days,serum was obtained.The serum was diluted into 5％ and 10％ concentrations with nutrient solution,was added into A549/DDP for 12h、24h、48h.The proliferation of A549/DDP cells was observed by MTT assay,and the reversal fold was computed.The expressions of E-cadherin and N-cadherin proteins were examined by immunohistochemistry and Western blot.Results Liu Junzi Decoction inhibited the growth and proliferation of 549/ DDP cells obviously,and with a concentration-dependent and time-dependent manner,10％ serum containing middle dose Liu Junzi decoction cultured for 48h has better effect and with no cell toxin,TGF-β 1 induced IC50 of A549/DDP cells decreased (P＜0.05),the reverse rate was 4.12.The serum containing Liu Junzi decoction increased the expression level of E-cadherin and reduced the expression level of N-cadherin.Conclusion The serum containing Liu Junzi decoction moderately reversed the multidrug resistance of A549/DDP cells,which probably is related to inhibiting epithelial mesenchymal transformation and increasing the sensitivity of A549/DDP to DDP.