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目的 探讨LPS刺激对血清淀粉样蛋白A转录激活因子 (SAF) 外显子e4B保留和剪切的影响.方法 LPS刺激不同时间THP-1细胞和去除刺激后放线菌素D处理THP-1细胞的cDNA作模板进行半定量和定量聚合酶链式反应.结果 LPS刺激2h SAF基因外显子e4B保留的转录子表达下降、e4B剪切的转录子表达增高, 延长刺激至24h, 二者的表达呈下降趋势.LPS刺激12h和24h后去除刺激给予放线菌素D初始, 上述转录子表达增高;延长放线菌素D作用至2h, 二者表达下降.结论 延长LPS刺激SAF基因外显子e4B保留和剪切的转录子表达下降可能和RNA降解有关.“,”Objective To investigate the effect of inflammation stimulation on the retention and splicing of exon 4B of SAF gene. Methods Total RNA was isolated by using TRIZol from THP-1 cells treated with LPS (10μg/mL) for 2h, 6h, 12h and 24h respectively. Then THP-1 cells were treated with 1μg/mL of transcription inhibitor actionmycin D and collected at 0. 5h, 1h and 2h for RNA isolation. DNase I treated RNA was used in the reverse-transcription reaction, c DNA was quantitated using PCR and RT-PCR. Results LPS decreased the retention of exon 4B of SAF transcripts and increased the splicing of exon 4B of SAF transcripts in THP-1 cells. The total throughput of SAF-1 mRNA and SAF-3 mRNA were increased with LPS for 2h. With the duration of the inflammatory stimulus to 24h, the exon 4B inclusion and exclusion of SAF transcripts were all reduced, that means the total throughput of SAF-1 mRNA and SAF-3 mRNA were also reduced. Upon actionmycin D after LPS treatment for 12h and 24h, the total throughput of SAF-1 mRNA, SAF-2 mRNA and SAF-3 mRNA actually increased at first. With the duration of actionmycin D to 2h, the exon 4B inclusion and exclusion of SAF transcripts were decreased rapidly. Conclusion The decreased retention and splicing of exon 4B of SAF transcripts might be related to RNA degradation during inflammation.