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岩黄连是罂粟科紫堇属植物(Corydalis saxicolaBunting),在广西民间用其根止痛消肿、拔毒、冶疗疥疮肿毒,后又用于治疗肝炎、肝硬化及肝癌。广西中医学院通过体外、半体内及体内法抗肿瘤试验,发现广西东兰制药厂提取的岩黄连总生物碱对小鼠肉瘤180、文氏腹水癌及肝癌腹水癌和大鼠癌肉瘤256均有一定的抑制作用。其总生物碱配制的注射液,经有关医疗单位对102例肝炎病例观察,总有效率为76.5%。为此,我们对生物碱的部位进行分离。取岩黄连全草(2kg)磨成粉,以90%酒精提取,得浸膏,用2%盐酸提取总生物碱,应用 pH 梯度分离,在强碱性部位分得小檗碱及 dehydrocavidine;弱碱性部位经硅胶低压柱层析及制备性薄层层析分得7
Rhizoma Coptidis is a plant of the genus Corydalis saxicola Bunting, which has been used to relieve swelling, detoxification, and cure boils in the Guangxi area. It is then used to treat hepatitis, liver cirrhosis and liver cancer. In Guangxi University of Traditional Chinese Medicine, anti-tumor experiments in vitro, half-body, and in vivo revealed that the total alkaloids extracted from Donglan Pharmaceutical Factory in Guangxi had all of 256 on mouse sarcoma 180, Wen’s ascites carcinoma, and ascites carcinoma of liver and rat carcinosarcoma 256. Certain inhibition. The total alkaloid injection preparation was observed by 102 cases of hepatitis cases, the total effective rate was 76.5%. For this purpose, we isolated the alkaloids. Take Huanglianliancao (2kg) ground into powder and extract with 90% alcohol to obtain extract. Use 2% hydrochloric acid to extract total alkaloids. Use pH gradient to separate and obtain berberine and dehydrocavidine in strongly alkaline sites. Alkaline parts were separated by silica gel column chromatography and preparative thin-layer chromatography. 7