Diversity Analysis in Selected Non-basmati Scented Rice Collection

• 作 者: Sarika MATHURE Narendra JAWALI Altafhusain NADAF
• 机 构: Department of Botany,University of Pune,Molecular Biology Division,Bhabha Atomic Research Centre,
• 出 处: Rice Science
• 发表日期: 2010年01期
• 关键词: rice random amplified polymorphic DNA inter-simple sequence repeat diversity lan

Diversity analysis among 23 rice varieties including 16 non-basmati scented accessions,5 basmati accessions and 2 non-scented accessions was performed by random amplified polymorphic DNA(RAPD) and inter-simple sequence repeat(ISSR) marker systems.The varieties analyzed by 11 RAPD and 8 ISSR primers yielded an average of 65% and 80% polymorphism,respectively.The average number of polymorphic bands generated per RAPD primer was 6 and per ISSR primer was 5.87.RAPD and ISSR data analysis individually could not segregate basmati and non-basmati scented rice accessions.However,the analysis using a combined data could group basmati and non-basmati scented rice accessions separately.The bands present specifically among three accessions of non-basmati scented rice were also identified.The study revealed a high genetic diversity among non-basmati scented rice accessions.

Diversity analysis among 23 rice varieties including 16 non-basmati scented accessions, 5 basmati accessions and 2 non-scented accessions was performed by random amplified polymorphic DNA (RAPD) and inter-simple sequence repeat (ISSR) marker systems. RAPD and 8 ISSR primers yielded an average of 65% and 80% polymorphism, respectively. The average number of polymorphic bands generated per RAPD primer was 6 and per ISSR primer was 5.87. RAPD and ISSR data analysis were could not segregate basmati and non- the analysis using a combined data could group group basmati and non-basmati scented rice accessions separately. the bands present specifically among three accessions of non-basmati scented rice were also identified. study revealed a high genetic diversity among non-basmati scented rice accessions.