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选用 40 0个随机寡核苷酸和 40个荧光标记的微卫星引物对 ,对我国主栽的协优杂交稻组合及其亲本进行多态性分析。RAPD分析显示 ,40 0个随机寡核苷酸引物中 ,有 36 4个能够在供试基因型材料中扩增出肉眼可见的 DNA条带 ,其中 2 8个显示扩增的多态性 ,这中间 9个引物产生的 13条多态性条带具有清晰、易分辨的特点 ,可以在供试的水稻组合与亲本之间 ,以及杂交组合之间进行区别和鉴定。微卫星分析指出 ,40对荧光标记引物中 ,13对引物产生的 31条标记带 ,能够在供试基因型材料中显示稳定的多态性 ,且杂种表现为双亲的互补型。与 RAPD相比 ,微卫星分析具有多态性强、精确性高和重复性好的特点。
Four hundred and forty random oligonucleotides and 40 fluorescently-labeled microsatellite primers were used to analyze the polymorphism of the Chinese hybrid combination Xieyou and its parents. RAPD analysis showed that 36 4 out of 400 random oligonucleotide primers were able to amplify macroscopic DNA bands in the genotypes tested, of which 28 showed amplified polymorphisms, which Thirteen polymorphic bands produced by the middle nine primers were clearly and easily distinguishable, which could be distinguished and identified between the tested rice combinations and their parents, as well as the crosses. Microsatellite analysis indicated that among the 40 pairs of fluorescently labeled primers, 31 bands generated by 13 pairs of primers could display stable polymorphism in the tested genotype materials and the hybrids showed the complement of parents. Compared with RAPD, microsatellite analysis has the characteristics of strong polymorphism, high accuracy and repeatability.