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目的:观察淋巴毒素β受体(LTβR)分子在系统性红斑狼疮(SLE)外周血单核细胞上的表达情况,同时探索能够影响其表达的因素。方法:分离SLE患者及正常健康对照者的外周血单个核细胞,流式细胞术检测LTβR分子在单核细胞上的表达,免疫磁珠法分选出单核细胞,RT-PCR方法检测其mRNA的表达;同时在体外培养细胞的U937和THP1中观察脂多糖(LPS)、佛波酯(PMA)及地塞米松(DEX)对LTβR分子表达的影响。结果:SLE患者外周血单核细胞上出现LTβR分子的异常高表达,而正常人外周血单核细胞上没有表达;体外细胞培养发现,地塞米松对U937细胞上的LTβR的表达具有上调作用,而对THP1细胞和正常对照者外周血单核细胞没有上调LTβR的表达;PMA可使U937和THP1细胞膜上LTβR出现一过性的降低,但不影响其mRNA水平,对正常人单核细胞无影响;LPS对U937和THP1细胞及正常人单核细胞的LTβR表达均无影响。结论:LTβR分子在SLE患者来源的外周血单核细胞上具有异常的表达,该分子的表达可能不是仅仅由于单核细胞活化或应用激素类药物引起的;但地塞米松可能对发育早期的单核细胞的LTβR表达具有一定的上调作用。
Objective: To observe the expression of lymphotoxin β receptor (LTβR) on peripheral blood mononuclear cells in patients with systemic lupus erythematosus (SLE) and explore the factors influencing its expression. Methods: Peripheral blood mononuclear cells were isolated from patients with SLE and normal controls. The expression of LTβR on monocytes was detected by flow cytometry. The mononuclear cells were sorted by immunomagnetic beads method. The mRNA was detected by RT-PCR (LPS), phorbol ester (PMA) and dexamethasone (DEX) on the expression of LTβR in cultured U937 and THP1 cells. Results: The aberrant expression of LTβR on peripheral blood mononuclear cells of SLE patients was abnormally high, but not on the peripheral blood mononuclear cells of normal people. In vitro cultured cells showed that dexamethasone up-regulated the expression of LTβR on U937 cells, However, the expression of LTβR in peripheral blood mononuclear cells of THP1 cells and normal controls did not increase. PMA decreased transiently the level of LTβR in U937 and THP1 cells, but did not affect the mRNA level and had no effect on normal human monocytes ; LPS had no effect on LTβR expression in U937 and THP1 cells and in normal human monocytes. CONCLUSIONS: LTβR molecules are aberrantly expressed on peripheral blood mononuclear cells derived from SLE patients. The expression of this molecule may not be due to activation of monocytes or the use of hormonal drugs alone. Dexamethasone, however, The expression of LTβR in nuclear cells has certain up-regulation effect.