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目的 探讨肿瘤坏死因子α(TNF α)对瘢痕成纤维细胞的生物学作用及其在异常瘢痕的发生和防治中的意义。方法 采用组织培养技术 ,建立了成纤维细胞系 ,以MTT法测定细胞活力 ,流式细胞仪检测纤维粘连蛋白 (FN)表达及间接免疫荧光法等技术和方法。对来源于增生性瘢痕和正常皮肤的成纤维细胞进行了对比研究。结果 ①高浓度的TNF α( 10 0 0U/ml ↑ )对增生性瘢痕成纤维细胞活力具有明显抑制作用 ,并使其FN表达减少 11 7% ;②TNF α对正常皮肤成纤维细胞活力具有明显促进作用 ,并使其FN表达增加 5 8%。结论 TNF α对于增生性瘢痕和正常皮肤的成纤维细胞具有不同的生物学作用。研究TNF对增生性瘢痕的调控机制 ,有可能为防治瘢痕的异常增生提供新的方法和途径。
Objective To investigate the biological effects of tumor necrosis factor α (TNF α) on scar fibroblasts and its significance in the occurrence and prevention of abnormal scar. Methods Tissue culture techniques were used to establish fibroblast cell lines. MTT assay was used to determine cell viability, flow cytometry to detect fibronectin (FN) expression and indirect immunofluorescence assay. Fibroblasts derived from hypertrophic scars and normal skin were compared. Results ① High concentration of TNFα (100μU / ml) significantly inhibited the viability of hypertrophic scar fibroblasts and decreased FN expression by 11.7%. ②TNFα significantly enhanced the viability of normal skin fibroblasts Role and increase its FN expression by 58%. Conclusion TNFα has different biological effects on fibroblasts of hypertrophic scars and normal skin. To study the regulatory mechanism of TNF on hypertrophic scars may provide new methods and approaches for preventing and treating scar dysplasia.