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建立了植物提取物中的碱性橙21、碱性橙22、碱性嫩黄O、罗丹明B、碱性橙2、酸性橙2等6种工业染料的液相色谱-串联质谱分析方法。样品经乙腈提取,固相萃取柱净化,使用Waters Acquity~(TM) UPLC BEH C18柱作为分析柱,以多反应监测(MRM)方式在ESI(±)模式下进行检测,外标法定量。结果表明:6种工业染料含量在5.0~50.0 ng/m L范围内线性良好,相关系数(r~2)均大于0.99;在5,10,25μg/kg 3个添加水平下回收率为65.0%~122.4%,相对标准偏差(RSD)在1.8%~12%(n=6)之间。方适用于植物提取物中多种工业染料的同时测定。
A liquid chromatography-tandem mass spectrometry (LC-MS / MS) method was developed for the determination of six commercial dyes, namely, alkaline orange 21, basic orange 22, basic yellow O, rhodamine B, basic orange 2 and acidic orange 2 in plant extracts. The samples were extracted with acetonitrile and cleaned up by solid-phase extraction (SPE) column. Waters Acquity UPLC BEH C18 column was used as analytical column and detected by ESI (±) mode with multiple reaction monitoring (MRM). The results showed that the linearity of the six industrial dyes in the range of 5.0-50.0 ng / m L was good, and the correlation coefficients (r ~ 2) were all greater than 0.99. The recoveries of three industrial dyes were 65.0% at 5, 10 and 25 μg / ~ 122.4%, relative standard deviation (RSD) between 1.8% ~ 12% (n = 6). Side for the simultaneous determination of a variety of industrial dyes in plant extracts.