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基于胰腺的胰胆固醇酯酶能在肠腔内水解胆固醇酰(1-~(13)C)辛醇的原理,已发展一种非侵入性的评价脂肪消化功能的试验方法。研究旨在确定基于此的呼气试验在评估胰腺外分泌功能中所起的诊断作用。 方法和对象:研究对象包括20例健康对照者,22例慢性胰腺疾病患者,4例胆胰转道术后(一种减肥手术)患者和32例其他消化道疾病患者。试餐内含胆固醇酰辛醇(1-~(13)C)和D-木糖(以评估胃对试餐的排空情况)。经12小时禁食后,收集试验对象的呼气,于试餐前及其后每隔15分钟1次,共收集6小时。以质谱仪测量~(13)C/~(12)C的同位素比值,并转换成δ~(13)C值(一种国际标准)。计算每小时回收的~(13)C占所给剂量的百分比。在1周内对部分对象重复本试验。在进行胆固醇酰辛醇呼气试验的2周内,
Based on the principle that pancreatic cholesterol esterase can hydrolyze cholesterol acyl (1- 13 C) octanol in the intestine, a noninvasive test method for evaluating the function of the adipose digest has been developed. The aim of the study was to determine the diagnostic value of the breath test based on this in evaluating pancreatic exocrine function. METHODS AND OBJECTIVES: Subjects included 20 healthy controls, 22 patients with chronic pancreatic disease, 4 patients undergoing cholangiopancreatography (a bariatric surgery) and 32 patients with other gastrointestinal diseases. The test meal contained cholesterol, octyl alcohol (1- 13 C) and D-xylose (to assess the emptying of the test meal). After 12 hours of fasting, subjects were exhaled breath, before and after the meal every 15 minutes, a total of 6 hours. The isotope ratios of ~ (13) C / ~ (12) C were measured by mass spectrometry and converted to δ ~ (13) C values (an international standard). Calculate the percentage of ~ (13) C recovered per hour for the dose given. This test was repeated on some subjects within one week. Within two weeks of the cholesterol acyl octanol breath test,