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目的 探讨白介素 8(IL 8)和干扰素α(IFN α)对体外培养的子宫内膜异位症患者的子宫内膜细胞的影响。方法 收集患者的子宫内膜组织进行体外培养、纯化 ,采用免疫组织化学的方法鉴定纯化的细胞。在培养基中加入不同浓度的IL 8(0 1、1、5、10、5 0ng/ml)和IFN α 2b(10、5 0、10 0、10 0 0、10 0 0 0U/ml) ,对腺上皮细胞进行培养 ,2 4h后用MTT法检测细胞增殖的状况。同时 ,用含有 1ng/mlIL 8的培养基对内膜细胞进行培养 ,2 4h后用透射电镜观察IL 8对腺上皮细胞和间质细胞的影响。结果 细胞的增殖与IL 8的含量有着明显的剂量依赖关系 ,并且在IL 8浓度为 10ng/ml时最为显著(P <0 0 5 )。电镜观察发现在 1ng/ml的IL 8的作用下 ,内膜细胞功能活跃 ,分泌旺盛 ,可见细胞外有胶原产生。而IFN α 2b对腺上皮细胞的增殖却有明显的抑制作用 (P <0 0 5 )。结论 本研究明确了IL 8和IFN α 2b对内膜细胞的影响 ,并且发现IL 8可以使内膜细胞分泌胶原纤维增多 ,这可能在子宫内膜异位症的发病机制中起着重要作用。IL 8可能是通过自分泌或者旁分泌的方式参与发病的。IFN α 2b是一类免疫正调因子 ,它可以调节子宫内膜异位症患者的免疫功能。免疫刺激疗法有可能成为治疗子宫内膜异位症的一种新的方法。
Objective To investigate the effects of interleukin 8 (IL 8) and interferon α (IFN α) on endometrial cells in patients with endometriosis. Methods The endometrial tissues of patients were collected and cultured in vitro. The purified cells were identified by immunohistochemistry. Different concentrations of IL8 (0 1, 1, 5, 10, 5 0 ng / ml) and IFNα 2b (10,5 0,10 0,10 0 0,10 0 0 0 U / ml) were added to the medium, The glandular epithelial cells were cultured, and the cell proliferation was detected by MTT method after 24 hours. In the meantime, intimal cells were cultured with medium containing 1 ng / ml IL8, and the effect of IL8 on glandular epithelial cells and interstitial cells was observed by transmission electron microscopy after 24 hours. Results The proliferation of cells was in a dose-dependent manner in a dose-dependent manner with respect to IL-8, and was most pronounced at a IL-8 concentration of 10 ng / ml (P <0 05). Electron microscopy showed that under the action of IL-8 at 1ng / ml, the intima cells were active and secreted, showing extracellular collagen. IFNα 2b had a significant inhibitory effect on the proliferation of glandular epithelial cells (P <0.05). Conclusions This study clarified the effect of IL 8 and IFNα 2b on endometrial cells, and found that IL 8 can increase collagen fibers secreted by endometrial cells, which may play an important role in the pathogenesis of endometriosis. IL 8 may be involved in the pathogenesis through autocrine or paracrine. IFN alpha 2b is a class of immunoreceptors that regulate the immune function of patients with endometriosis. Immunostimulatory therapy may become a new method of treatment of endometriosis.