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[目的 ]筛选旋毛虫肌幼虫可溶性抗原中具有免疫显性的表位。 [方法 ]采用杂交瘤技术 ,获得 15株特异性单克隆抗体 ,随后用酶联免疫吸附试验 (ELISA)、免疫印迹法 (Westernblotting)和间接免疫荧光试验(IFA)对部分免疫显性抗原进行分析。 [结果 ]Westernblotting试验显示 ,6株单抗与旋毛虫肌幼虫可溶性抗原反应显示有特异条带 ,分子量为 40～ 70kDa ;而多抗血清则可识别 2 0～ 2 0 0kDa之间 10条条带。IFA可观察到 ,6株单抗中有 4株单抗的靶抗原定位在旋毛虫肌幼虫表皮层上 ,另 2株定位于杆状体 (stichosome)及表皮层。 [结论 ]识别与分析部分旋毛虫肌幼虫可溶性抗原中具有免疫显性的表位 ,为纯化旋毛虫的抗原及疫苗靶抗原的研制提供了有价值的实验依据。
[Objective] The research aimed to screen immunodominant epitopes in the soluble antigens of Trichinella spiralis larvae. [Method] Fifteen specific monoclonal antibodies were obtained by hybridoma technique. Subsequently, some immunodominant antigens were analyzed by ELISA, Western blotting and indirect immunofluorescence assay (IFA) . [Results] The results of Western blotting showed that the soluble antigens of six monoclonal antibodies against Trichinella spiralis larvae showed specific bands with the molecular weight of 40-70 kDa, while the polyclonal antiserum could recognize 10 bands of 20-200 kDa . IFA observed that the target antigens of 4 monoclonal antibodies in 6 monoclonal antibodies were located on the epidermis of Trichinella spiralis larvae and the other 2 were located on the stichosome and epidermis. [Conclusion] Identification and analysis of some immunodominant epitopes in the soluble antigen of Trichinella spiralis larvae provided valuable experimental evidence for the development of antigens of Trichinella spiralis and vaccine target antigen.