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Background The expression of syndecan-1 after myocardial infarction (MI) is not consistent. In this study, we investigated the changes in the cxpression of synolecan-1 and the relationship between soluble syndeean-1 and syndecan-1 expressed in myocytes after MI. Methods Spreague-Dawley rats survived 24 hours after ligating left anterior descending coronary artery were randomly divided into four groups: MI 1 day group, MI 3 days group, MI 7 days group and MI 14 days group. A Sham-operated group was selected as non-infarcted control. There were 6 rats in each group. At the end of observation the expressions of syndecan-1 mRNA (Realtime RT-PCR) and protein (Western blot) in the peri-infarct myocardium and soluble syndecan-1 in serum (ELISA) were detected. Results There were minimal syndecan-1 mRNA, protein and soluble syndecan-1 expressed in sham-operated rats. The expressions of syndecan-1 mRNA, protein and soluble syndecan-1 increased progressively after MI, reaching their maximum on the 3rd day and decreasing again after that. There were statistically significant differences between each groups (P < 0.01). Pearson correlation analysis showed that the concentration of soluble syndecan-1 in serum was positively correlated with syndecan-1 expressed in peri-infarct myocardium (r = 0.952, P < 0.01). Conclusions The expression of syndecan-1 increases and reaches its maximum on the 3rd day after MI . The concentration of soluble syndecan-1 in serum can reflect the level of syndecan-1 expressed in peri-infarct myocardium to some extent.
Background The expression of syndecan-1 after myocardial infarction (MI) is not consistent. In this study, we investigated the changes in the cxpression of synolecan-1 and the relationship between soluble syndeean-1 and syndecan-1 expressed in myocytes after MI. Methods Spreague-Dawley rats survived 24 hours after ligating left anterior descending coronary artery were randomly divided into four groups: MI 1 day group, MI 3 days group, MI 7 days group and MI 14 days group. A Sham-operated group was selected as There are 6 rats in each group. At the end of observation the expressions of syndecan-1 mRNA (Realtime RT-PCR) and protein (Western blot) in the peri-infarct myocardium and soluble syndecan-1 in serum (ELISA) were detected. Results There were minimal syndecan-1 mRNA, protein and soluble syndecan-1 expressed in sham-operated rats. The expressions of syndecan-1 mRNA, protein and soluble syndecan-1 increased progressively after MI, reached their maximum on th e 3rd day and decreasing again after that. There were statistically significant differences between each groups (P <0.01). Pearson correlation analysis showed that the concentration of soluble syndecan-1 in serum was positively correlated with syndecan-1 expressed in peri-infarct myocardium Conclusions The expression of syndecan-1 increases and reaches its maximum on the 3rd day after MI. The concentration of soluble syndecan-1 in serum can reflect the level of syndecan-1 expressed in peri- infarct myocardium to some extent.