论文部分内容阅读
目的在佐剂性关节炎大鼠模型基础上建立关节炎大鼠慢性吗啡耐受模型,观察兴奋性氨基酸载体1(EAAC1)在关节炎慢性吗啡耐受大鼠的脊髓背角表达的变化,探讨EAAC1在吗啡耐受形成机制中的作用。方法将36只健康雄性SD大鼠随机分为6组(n=6),行鞘内置管。其中4组制成佐剂性关节炎模型,分别经鞘内给予生理盐水(A组)、吗啡10μg(B组)、吗啡20μg(C组)、吗啡10μg加纳洛酮10μg(D组),另外两组非致炎大鼠分别经鞘内给予生理盐水(E组)、吗啡20μg(F组)。各组给药均为1日2次,连续7d。用VonFrey丝动态检测大鼠50%机械缩爪阈值,分别以免疫组化和Westernblot方法检测各组大鼠给予吗啡后脊髓背角EAAC1表达。结果B、C两组关节炎大鼠在鞘内给予吗啡后第7天形成较稳定的机械痛敏,标志关节炎大鼠慢性吗啡耐受模型建立成功,其脊髓背角EAAC1的表达下调。结论脊髓EAAC1可能参与炎性痛大鼠慢性吗啡耐受的形成机制。
OBJECTIVE: To establish chronic morphine tolerant model of arthritis rats on the basis of adjuvant arthritis rat model and to observe the changes of EAAC1 expression in spinal cord dorsal horn of chronic morphine tolerant arthritis rats The Role of EAAC1 in the Mechanism of Morphine Tolerance. Methods Thirty-six healthy male Sprague-Dawley rats were randomly divided into 6 groups (n = 6). Four groups were given adjuvant arthritis model. The rats in group A were given intrathecal saline (group A), morphine 10μg (group B), morphine 20μg (group C), morphine 10μg ganaxone 10μg (group D) Two groups of non-inflammatory rats were administered with saline (group E) and morphine 20μg (group F) respectively. Each group were administered twice a day for 7 days. Dynamic threshold of 50% mechanical paw withdrawal in rats was measured by Von Frey wire. The expression of EAAC1 in spinal dorsal horn of morphine rats was detected by immunohistochemistry and Western blot respectively. Results The rats in groups B and C developed stable mechanical hyperalgesia on the 7th day after the intrathecal administration of morphine, which marked the successful establishment of chronic morphine tolerance model in rats with arthritis. The expression of EAAC1 in the spinal dorsal horn was down-regulated. Conclusion Spinal cord EAAC1 may be involved in the mechanism of chronic morphine tolerance in inflammatory pain rats.