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目的:探讨全自动DNA倍体分析系统在宫颈癌早期诊断中的应用价值。方法:对参与宫颈癌普查的92名妇女进行液基薄层制片,分别进行巴氏染色和Feulgen染色,由细胞学医师对巴氏染色片做常规细胞学诊断,应用全自动DNA倍体分析系统对Feulgen染色片进行自动扫描诊断。结果:以病理诊断结果为标准,计算常规细胞学诊断和细胞DNA倍体分析方法在筛查宫颈上皮内瘤变Ⅱ级(CINⅡ)及以上宫颈病变的敏感性、特异性、阳性预测值、阴性预测值。常规细胞学检查为低级别鳞状上皮内病变(LSIL)及以上级别评估CINⅡ及以上病理改变标准,其敏感性、特异性、阳性预测值、阴性预测值分别是37.04%、69.23%、33.33%、72.58%;DNA倍体分析中以≥3个DNA异倍体作为评估CINⅡ及以上病理改变,其敏感性、特异性、阳性预测值、阴性预测值分别是77.78%、66.15%、50.00%、86.00%。在ASC-US分流方面有一定的临床指导价值。结论:全自动DNA倍体分析系统能够较好地提高宫颈癌普查的阳性检出率。
Objective: To investigate the value of automatic DNA ploidy analysis system in the early diagnosis of cervical cancer. Methods: Ninety-two women who participated in the cervical cancer screening were divided into liquid-based thin-layer film, respectively, Papanicolaou staining and Feulgen staining. Cytological diagnosis of Pap smear was done by cytology physicians. The system automatically diagnoses Feulgen stains. Results: According to pathological diagnosis, the sensitivity, specificity, positive predictive value and negativeness of routine cytological diagnosis and DNA ploidy analysis in screening cervical intraepithelial neoplasia grade Ⅱ (CINⅡ) and above cervical lesions were calculated Predictive value. The sensitivity, specificity, positive predictive value and negative predictive value were 37.04%, 69.23%, 33.33% respectively for routine cytological examination of low-grade squamous intraepithelial lesion (LSIL) and above grade for CINⅡ and above pathological changes , 72.58% respectively. The sensitivity, specificity, positive predictive value and negative predictive value of ≥3 DNA aneuploidy in CINⅡ and pathological changes were 77.78%, 66.15%, 50.00% 86.00%. In the ASC-US shunt has some clinical value. Conclusion: The automatic DNA ploidy analysis system can improve the positive detection rate of cervical cancer screening.