论文部分内容阅读
本实验采用RFLP技术,对中国东部栗疫病菌(Cryphonectria parasitica)进行了群体遗传结构的研究。313个参试菌株来自10个省(市)的16个群体(子群体),样本分布在北纬24°N—41°N。各菌株的DNA分别用限制性内切酶Pst Ⅰ和EcoR Ⅰ酶切,先后以10个低拷贝DNA探针和1个DNA指纹图谱探针进行了杂交和检测。结果表明,两个探针(pCB29和pMS29.1)的杂交图谱呈单态性;探针pCB19的杂交图谱显示,菌株DNA以PstⅠ酶切的为单态性,以EcoR Ⅰ酶切的则呈多态性;其他7个低拷贝探针的杂交图谱都呈多态性(Pst Ⅰ酶切)、指纹图谱探针的检测结果显示,辽宁凤城群体的菌株与中国东部其他群体的菌株相比,具有更多的限制性杂交片段,菌株间的遗传变异性也更大。
In this study, population genetic structure of Cryphonectria parasitica was studied using RFLP technique. The 313 tested strains were from 16 populations (subpopulations) in 10 provinces (cities) and the samples were located at latitude 24 ° N-41 ° N. The DNA of each strain was digested with restriction endonucleases Pst I and EcoR I respectively, and then hybridized and detected with 10 low copy DNA probes and 1 DNA fingerprinting probe. The results showed that the hybridization patterns of the two probes (pCB29 and pMS29.1) were single. The hybridization of pCB19 showed that the DNA of the strain was single-stranded with PstⅠ and was digested with EcoR Ⅰ The polymorphism of the other 7 low-copy probes was polymorphic (PstI digestion). The results of fingerprinting probe showed that the strains of Fengcheng in Liaoning Province were compared with those of other populations in eastern China , With more restricted hybridization fragments, the genetic variability between strains is also greater.