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目的通过对公主岭市不同人群丙型肝炎病毒(HCV)抗体初筛检测和对初筛抗-HCV阳性血清进行丙型肝炎病毒核糖核酸(HCV RNA)定性检测,了解本地区人群HCV感染分布和丙型肝炎病毒的实验室诊断情况,为预防丙型肝炎和丙型病毒性肝炎的实验室诊断提供科学依据。方法第三代抗HCV酶联免疫吸附试验(ELISA)进行抗-HCV初筛检测,逆转录-多聚酶链反应(RT-PCR)法进行丙型肝炎病毒核糖核酸定性检测。结果 3 873例检测样本检出抗-HCV阳性166例,阳性率为4.29%;抗-HCV阳性样本HCV RNA阳性105份,阳性率63.25%(105/166)。结论本地区丙型病毒性肝炎感染率较国内略高,应全面加强丙型肝炎知识的宣传,提高丙型病毒性肝炎的实验室筛查率;HCV RNA检测结果提示丙型肝炎病毒(HCV)抗体初筛检测阳性的人群中63.25%处于HCV感染活动期,各级医疗机构有必要对抗-HCV阳性者进行HCV RNA确认,以提高丙型病毒性肝炎的临床诊断与治疗。
Objective To investigate the distribution of HCV infection in population in this area by screening the hepatitis C virus (HCV) antibody of different population in Gongzhuling city and qualitative screening of hepatitis C virus (HCV RNA) in primary anti-HCV positive sera Hepatitis B virus laboratory diagnosis, in order to prevent hepatitis C and hepatitis C laboratory diagnosis provides a scientific basis. Methods The third generation anti-HCV enzyme-linked immunosorbent assay (ELISA) was used to detect the anti-HCV virulence and the qualitative detection of hepatitis C virus RNA was detected by reverse transcription-polymerase chain reaction (RT-PCR) Results A total of 166 positive anti-HCV antibodies were detected in 3 873 samples. The positive rate was 4.29%. The anti-HCV positive samples were 105 positive for HCV RNA, the positive rate was 63.25% (105/166). Conclusion The prevalence of hepatitis C virus in China is slightly higher than that in China. The publicity of hepatitis C knowledge should be strengthened and the laboratory screening rate of hepatitis C virus should be increased. HCV RNA test results suggest that hepatitis C virus (HCV) 63.25% of the population with positive antibody screening test are in active stage of HCV infection, and it is necessary for medical institutions at all levels to confirm the HCV RNA in HCV-positive patients so as to improve the clinical diagnosis and treatment of hepatitis C virus.