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目的 研究紫杉醇对成骨肉瘤细胞系U2 OS的生长抑制及凋亡诱导作用。方法 将不同浓度的紫杉醇作用于成骨肉瘤细胞系U2 OS ,应用形态学观察、台盼蓝染色、流式细胞术、电镜、原位末端标记 (TUNEL)等方法 ,检测其诱导骨肉瘤细胞凋亡的时间效应和剂量效应 ,并与其他化疗药物进行对比。结果 紫杉醇对U2 OS细胞的生长抑制及凋亡诱导作用具有明显的时间依赖和剂量依赖性。细胞周期分析出现G2 /M期阻滞和明显的凋亡峰。形态学检查示核碎裂及染色体凝集的凋亡特征 ,出现大量多核细胞。经其他化疗药物诱导的U2 OS细胞未出现多核现象。免疫组织化学检测示紫杉醇诱导后细胞核中有广泛DNA断裂结论 紫杉醇通过抑制骨肉瘤细胞有丝分裂发挥抑制细胞生长及促进细胞凋亡的作用 ,该作用具有时间依赖和剂量依赖性
Objective To study the growth inhibition and apoptosis induction of paclitaxel on osteosarcoma cell line U2 OS. Methods Different concentrations of paclitaxel were applied to the osteosarcoma cell line U2 OS. Morphological observation, trypan blue staining, flow cytometry, electron microscopy and TUNEL were used to detect the apoptosis of osteosarcoma cells Time-effects and dose-response effects were compared with other chemotherapeutic agents. Results paclitaxel on U2 OS cell growth inhibition and apoptosis induced by a significant time-dependent and dose-dependent manner. Cell cycle analysis showed G2 / M arrest and significant apoptotic peak. Morphological examination revealed apoptotic features of nuclear fragmentation and chromosomal agglutination with a large number of multicellular nuclei. U2OS cells induced by other chemotherapeutic drugs did not appear to have multi-nuclear phenomenon. Immunohistochemical detection showed extensive DNA breaks in the nucleus after paclitaxel induction. Conclusions Paclitaxel can inhibit cell growth and promote apoptosis by inhibiting the mitosis of osteosarcoma cells in a time-and dose-dependent manner