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目的探讨嗜铬蛋白A在哮喘发病机制中的作用。方法通过哮喘大鼠模型,分别采用免疫组化法和实时荧光定量PCR法检测肺组织和血中性粒细胞(PMN)嗜铬蛋白A或mRNA的表达。结果哮喘组肺组织嗜铬蛋白A及mRNA的表达水平显著高于对照组,差异有统计学意义(P<0.01);布地奈德组嗜铬蛋白A及mRNA的表达水平显著低于哮喘组,差异有统计学意义(P<0.01)。哮喘组血PMN嗜铬蛋白A mRNA的表达量显著高于对照组,差异有统计学意义(P<0.01),布地奈德组嗜铬蛋白A mRNA的表达量与哮喘组差异无统计学意义(P>0.05)。结论哮喘大鼠嗜铬蛋白A的表达升高,嗜铬蛋白A可能参与了哮喘的炎症机制,其表达能被布地奈德所抑制。
Objective To investigate the role of Chromogranin A in the pathogenesis of asthma. Methods The expression of Chromogranin A or mRNA in lung tissue and blood neutrophil (PMN) were detected by immunohistochemistry and real-time fluorescence quantitative PCR respectively in asthmatic rat model. Results The expressions of Chromogranin A and mRNA in the lung tissue of asthma group were significantly higher than those in the control group (P <0.01). The levels of Chromogranin A and mRNA in the budesonide group were significantly lower than those in the asthma group The difference was statistically significant (P <0.01). The expression level of chromogranin A mRNA in the asthmatic group was significantly higher than that in the control group (P <0.01), while there was no significant difference between the expression of Chromogranin A mRNA and the asthma group in the budesonide group P> 0.05). Conclusion The expression of Chromogranin A in asthmatic rats is elevated. Chromogranin A may be involved in the inflammatory mechanism of asthma and its expression can be inhibited by budesonide.