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目的:建立山莨菪根的HPLC指纹图谱,为其药材质量控制提供一种可靠的方法。方法:采用Ultimate AQ C18(250 mm×4.6 mm,5μm)色谱柱;以乙腈-10 mmol/L KH2PO4缓冲液(用H3PO4调节p H至3.0)为流动相梯度洗脱;流速1.0 m L/min;柱温30℃;检测波长210 nm;进样量10μL。并对测得指纹图谱进行相似度评价和主成分分析。结果:18批不同山莨菪根样品共确立了15个共有峰,建立了山莨菪根的HPLC对照指纹图谱,结合保留时间和紫外光谱,指认了樟柳碱、东莨菪碱、山莨菪碱、阿托品4个特征峰。18批野生山莨菪根的相似度在0.891~0.987之间。对15个共有峰进行主成分分析,综合评判得分在-0.85~0.89之间。结论:所建立的指纹图谱具有良好的精密度、重复性和稳定性,为山莨菪根的鉴别和质量控制提供了更全面的信息。
OBJECTIVE: To establish HPLC fingerprints of anisodus macrophylla and provide a reliable method for its quality control. Methods: The column was eluted with Ultimate AQ C18 (250 mm × 4.6 mm, 5 μm). The mobile phase was eluted with 10 mmol / L KH2PO4 buffer (adjusted to pH 3.0 with H3PO4). The flow rate was 1.0 mL / min ; Column temperature 30 ℃; detection wavelength 210 nm; injection volume 10μL. The fingerprint similarity was evaluated and the principal component analysis was carried out. Results: Fifteen common peaks were identified in 18 batches of anisodanas. HPLC fingerprints of anisodamine were established. According to the retention time and ultraviolet spectrum, anisodine, scopolamine, anisodamine and atropine were identified Characteristic peaks. The similarity of 18 batches of wild Anisodus scoparia was between 0.891 and 0.987. The principal component analysis of 15 common peaks, comprehensive evaluation score between -0.85 ~ 0.89. Conclusion: The established fingerprint has good precision, repeatability and stability, and provides more comprehensive information for the identification and quality control of Anisodysius.