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目的 探讨丙型肝炎病毒核心基因 (hepatitisCviruscoregene ,HCVC基因 )调控端粒酶逆转录酶(humantelomerasereversetranscriptase,hTERT)基因表达在肝门部胆管癌发生中的作用。方法 将构建成功的HCVC基重组真核表达载体pcDNAHCV -C和空载体与绿色荧光蛋白基因共转染人胆管正常上皮细胞 (hu mannormalbiliaryepithelialcells ,BEC)和胆管癌细胞QBC939,RT -PCR和免疫组织化学方法分析转染后细胞内hTERTmRNA和蛋白的表达。结果 pcDNAHCV -C在BEC和QBC939细胞中的转染率为 16 %和 32 % ,转染HCVC基因的BEC组表达hTERTmRNA ,而转染空载体的BEC不表达hTERTmRNA ;表达HCVC蛋白QBC939中hTERTmRNA和蛋白的表达明显高于空载体组。结论 转染的HCVC基因可以上调hTERT基因的表达 ,可能与肝门部胆管癌发生有关
Objective To investigate the role of hepatitis C virus core gene (HCVC) in the regulation of hTERT gene expression in hilar cholangiocarcinoma. Methods The constructed HCVC recombinant eukaryotic expression vector pcDNAHCV-C and empty vector and green fluorescent protein gene were co-transfected into human normal biliary epithelial cells (BEC) and cholangiocarcinoma cells QBC939, RT-PCR and immunohistochemistry Methods The expression of hTERT mRNA and protein in transfected cells was analyzed. Results The transfection rates of pcDNAHCV-C in BEC and QBC939 cells were 16% and 32%, respectively. The BEC group transfected with HCVC gene expressed hTERT mRNA while the BEC transfected with empty vector did not express hTERT mRNA. The expression of hTERT mRNA and protein in HCVBC protein QBC939 The expression was significantly higher than the empty vector group. Conclusion Transfection of HCVC gene can up-regulate the expression of hTERT gene, which may be related to the occurrence of hilar cholangiocarcinoma