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AIM:The finding that some Helicobacter pyloristrains expressLewis b(Le~b)blood group antigen casts a doubt on the roleof Le~b of human gastric epithelium being a receptor for H.pylori.The aim of this study was to determine if expressionof Le~b in H.pylori interferes with bacterial adhesion property.METHODS:Bacterial adhesion to immobilized Le~b onmicrotitre plate was performed in 63 H.pyloristrains obtainedfrom Singapore using in vitro adherence assay.Expression ofLewis blood group antigens was determined by ELISA assay.RESULTS:Among 63 H.pylori strains,28 expressed Le~bantigen.In vitro adhesion assay showed that 78.6%(22/28)of Le~b-positive and 74.3%(26/35)of Le~b-negative H.pyloriisolates were positive for adhesion to immobilized Le~bcoated on microtitre plate(P=0.772).In addition,blockingof H.pylori Le~b by prior incubation with anti-Le~b monoclonalantibody did not alter the binding of the bacteria to solid-phase coated Le~b.CONCLUSION:The present study suggests that expressionof Le~b in H.pylori does not interfere with the bacterialadhesion property.This result supports the notion that Le~bpresent on human gastric epithelial cells is capable of beinga receptor for H.pylori.
AIM: The finding that some Helicobacter pyloristrains express Lewis b (Le ~ b) blood group antigen casts a doubt on the role of Le ~ b of human gastric epithelium being a receptor for H. pylori. The aim of this study was to determine if expression of Le ~ b in H. pylori interferes with bacterial adhesion property. METHODS: Bacterial adhesion to immobilized Le ~ b on microtiter plate was performed in 63 H. pyloristrains obtained from Singapore using in vitro adherence assay. Expression of Lewis blood group antigens was determined by ELISA assay .RESULTS : Among 63 H. pylori strains, 28 expressed Le ~ bantigen.In vitro adhesion assay showed that 78.6% (22/28) of Le ~ b-positive and 74.3% (26/35) of Le ~ b-negative H. pyloriisolates were positive for adhesion to immobilized Le ~ bcoated on microtitre plate (P = 0.772). Addition, blocking of H. pylori Le ~ b by prior incubation with anti-Le ~ b monoclonalantibody did not alter the binding of the bacteria to solid-phase coated Le ~ b.CONCLUSION: The present study suggests that expressionof Le ~ b in H.pylori does not interfere with the bacteria growth property. This result supports the not that that Le ~ bpresent on human gastric epithelial cells is capable of being a receptor for H. pylori.