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利用菌丝生长速率法和孢子萌发法研究顶孢霉代谢产物对玉米圆斑病菌生长的抑制能力。结果表明,顶孢霉发酵液对玉米圆斑病菌分生孢子萌发和菌丝生长均有抑制作用。菌丝生长速率测定表明,含有顶孢霉菌代谢产物的培养基(D+P)圆斑病菌菌丝的生长速率低于含有玉米圆斑病菌代谢产物的培养基(Y+P)和空白对照PDA的生长速率,菌丝生长速率分别为65.0、86.5、87.5 mm/d;以Y+P和PDA为对照,D+P对玉米圆斑病菌在第1天和第2天时抑制率最高,均达58%以上;在1 mL PDA体系中,顶孢霉发酵液添加量为0.5 mL时,抑制率可达100%,不同浓度处理间及其与对照处理间顶孢霉发酵液抑制率差异显著(P=0.05);同一处理不同培养天数顶孢霉发酵液抑制率也有显著差异(P=0.05)。孢子萌发实验表明,经顶孢霉发酵液处理后的玉米圆斑孢子萌发率降低,在发酵液浓度为0.9 mL/mL时,萌发率仅为24%,抑制率可达66.67%,EC50约为68 mL/100 mL,且孢子经发酵液处理后产生畸形,不能正常发育。
The mycelium growth rate method and spore germination method were used to study the inhibitory effect of the metabolites of Acremonium sp. On the growth of Acorus sclerotiorum. The results showed that the fermentation liquid of Acaryticercus could inhibit conidial germination and mycelium growth of S. turcica. The mycelial growth rate showed that the mycelial growth rate of C. hyphae culture medium containing Acremonium metabolites was lower than that of the medium containing Y. pilosula metabolites (Y + P) and the blank control PDA The growth rate of mycelia were 65.0,86.5 and 87.5 mm / d, respectively. When Y + P and PDA were used as control, D + P had the highest inhibitory rate against S. turcica on day 1 and day 2 58%. When the amount of Acremonium fermentation broth was 0.5 mL in 1 mL PDA system, the inhibition rate was up to 100%. The inhibition rates of Acremonium fermentation broth between different treatments and control treatments were significantly different P = 0.05). There was also significant difference (P = 0.05) in inhibition rate of Acremonium fermentation broth in the same treatment day. The spore germination experiment showed that the spore germination rate decreased after Acremonium fermentation broth was treated. When the fermentation broth was 0.9 mL / mL, the germination rate was only 24% and the inhibition rate was 66.67%. The EC50 was about 68 mL / 100 mL, and spores produced by the fermentation broth after deformity, can not normally develop.