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肺结核患者由于长期使用抗痨药物,造成免疫功能低下,可能会引起肝炎病毒的合并感染,我们对本院收治的120例肺结核患者血清进行了HEV-IgM抗体的检测。现将结果报道如下。 1 材料与方法 1.1 标本来源标本取自本院1993年1月～1995年8月收治的120例肺结核患者,其甲型、乙型、丙型、丁型肝炎血清学指标均阴性。方法和结果判定按说明书进行。 1.2 使用美国Genelabs公司下属的新加坡Diagnostic Biotechnology公司的产品,抗-HEV-IgM ELISA。 1.3 仪器比利时Reader 510酶标仪。 2 结果 2.1 临界值的判定检测38例健康献血员的血清抗-HEV-IgM全部阴性,其吸光度(A)范围在0.002～0.287,(?)=0.076,s=0.065,(?)+5s=0.401,故确定临界值为0.4阳性,阴性对照A值,与试剂盒提供的判定
Tuberculosis patients due to long-term use of anti-tuberculosis drugs, resulting in immune dysfunction, may cause hepatitis virus co-infection, we treated 120 patients with tuberculosis in our hospital serum HEV-IgM antibody detection. The results are reported below. 1 Materials and Methods 1.1 Specimens from the hospital from January 1993 to August 1995 admitted 120 cases of tuberculosis patients, and its serological indicators of hepatitis A, B, C and D were negative. Method and result of judgment according to the instructions. 1.2 using the United States Genelabs company under the Singapore Diagnostic Biotechnology company’s products, anti-HEV-IgM ELISA. 1.3 Instruments Belgian Reader 510 microplate reader. 2 Results 2.1 Determination of the cutoff value The serum anti-HEV-IgM of 38 healthy blood donors was negative. The absorbance (A) ranged from 0.002 to 0.287 (?) = 0.076, s = 0.401, so determine the critical value of 0.4 positive, negative control A value, and the kit provides the decision