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目的 研究 p1 6基因在人肺癌中表达和 DNA倍体的关系及意义。方法 以免疫组化S- P法检测 82例肺癌组织中 p1 6蛋白表达 ,用图像分析仪测定 DNA倍体。结果 p1 6在肺癌表达阳性率为 51 .2 % ,低于癌旁组织 (P<0 .0 5) ,腺癌 p1 6阳性率最高为 68.8% ,高于鳞癌及未分化癌(P<0 .0 5)。在有淋巴结转移和无淋巴结转移组间 、 期与 、 期两组间 ,p1 6表达阳性率差异均有显著性 (P<0 .0 1 )。同时发现二倍体 p1 6阳性率显著高于异倍体 (P<0 .0 5)。结论 p1 6基因异常与肺癌发生发展有关 ,在不同组织类型肺癌发生中的作用不同 ,且与肺癌临床分期及恶性程度有关。
Objective To study the relationship between p16 gene expression and DNA ploidy in human lung cancer and its significance. Methods Immunohistochemical S-P method was used to detect the expression of p16 protein in 82 cases of lung cancer. DNA ploidy was measured by image analyzer. Results The positive expression rate of p16 in lung cancer was 51.2%, which was lower than paraneoplastic tissue (P<0.05). The highest positive rate of p16 in adenocarcinoma was 68.8%, higher than that in squamous cell carcinoma and undifferentiated carcinoma (P< 0 .0 5). There was a significant difference in the positive rate of p16 expression between the two groups with lymph node metastasis and no lymph node metastasis between the two groups (P < 0.01). At the same time, the positive rate of diploid p16 was significantly higher than that of aneuploid (P<0.05). Conclusion The abnormality of p16 gene is related to the occurrence and development of lung cancer. The role of p16 gene mutation in different types of lung cancer is different, and it is related to the clinical stage and malignancy of lung cancer.