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一般认为,在哺乳动物卵巢,PGE_2通过 cAMP间接发挥其类固醇合成作用。已发现在所有试验动物,PGE_2使 cAMP 增加,但依种属不同,作用方式也不同,即:在有的种属,PGE_2通过与受体特异结合,激活腺苷酸环化酶,有的则不然。作者用各种不同的酶处理大鼠和牛卵巢膜,确定由PGE_2引起的 cAMP 积聚作用。实验中将组织清洗、剪碎、并做成匀浆,部分用酶处理,在37℃下孵育30分,然将一定量匀浆物加入培养基质
It is generally believed that PGE 2 indirectly exerts its steroidogenic effects via cAMP in mammalian ovaries. It has been found that PGE 2 increases cAMP in all animals tested, but differs depending on species and species. In some species, PGE 2 activates adenylate cyclase through specific binding to receptors, while others otherwise. The authors used a variety of different enzymes to treat rat and bovine ovarian membranes to determine the accumulation of cAMP caused by PGE2. In the experiment, the tissue was washed, sheared, homogenized and partially treated with enzyme and incubated at 37 ° C for 30 minutes. Then, a certain amount of homogenate was added to the culture medium