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草莓花药培养,以MS为基本培养基添加BA0.5 毫克/升+ 2,4- D0.25 毫克/升,愈伤组织诱导率达129.17% ;添加BA2.0 毫克/升+ 2,4- D1.0 毫克/升,诱导率125% 。同一培养基,不同基因型草莓品种诱导率相差很大,“西班牙”的愈伤组织诱导率比“丰庆”提高82.29% 。草莓花蕾直径在3～5 毫米、花粉发育为单核期的诱导率比花蕾直径在6 毫米以上、花粉发育为初双核期的高6 倍左右。
Strawberry anther culture, MS medium supplemented with BA 0.5 mg / L + 2,4- D 0.25 mg / L, callus induction rate of 129.17%; BA 2.0 mg / L + 2,4- D1.0 mg / L, induction rate of 125%. The same medium, different genotypes of strawberry varieties induction rate vary greatly, “Spain” callus induction rate than “Fengqing” increased 82.29%. Strawberry bud diameter of 3 to 5 mm, the development of pollen mononuclear bud induction than the diameter of 6 mm or more, pollen development of the early dual-core period of about 6 times higher.