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研究在中国仓鼠卵巢（ＣＨＯ）细胞中稳定表达的δ阿片受体（ＤＯＲ）内吞现象及其Ｃ－末端在受体内吞中的作用．方法：用免疫荧光共聚焦显微镜显示受体的着膜；以耐酸性缓冲液洗涤法测定放射性配体－受体结合量确定受体内吞程度．结果：表达的野生（ＣＨＯ－Ｗ）和Ｃ－末端截短（ＣＨＯ－Ｔ）的ＤＯＲ都被正确转运到细胞膜上，激动剂［３Ｈ］亮氨酸－２－丙氨酸脑啡肽（ＬＡＥ）孵育诱导ＤＯＲ快速内吞，拮抗剂［３Ｈ］二丙诺啡（Ｄｉｐ）则不能；ＤＯＲ内吞对胞外高渗透压和温度敏感，但不受百日咳毒素和广谱蛋白激酶抑制剂星形孢菌素的影响；Ｃ－末端截短的ＤＯＲ在ＣＨＯ－Ｔ中的最大内吞不变，但增加较缓慢，结论：ＤＯＲ内吞是一种衣被小泡和激动剂依赖的过程，但不需受体后信号传导．Ｃ－末端与受体的胞膜锚定无关，但可影响受体内吞的初始化。
To investigate the endocytosis of δ opioid receptor (DOR) stably expressed in Chinese hamster ovary (CHO) cells and its C-terminal endocytosis. Methods: The immunofluorescence confocal microscope was used to display the receptor membrane; the amount of radioligand-receptor binding was determined by acid-fast buffer washing method to determine the extent of receptor endocytosis. Results: The expressed DOR of both wild (CHO-W) and C-terminal truncations (CHO-T) was correctly transported to the cell membrane and the agonist [3H] leucine-2-alanine enkephalin ) Did not induce rapid endocytosis of DOR, antagonist [3H] diprenaline (Dip) did not; DOR endocytosis was sensitive to extracellular hypertonicity and temperature but not to pertussis toxin and broad-spectrum protein kinase inhibitor star The effect of cyclosporine on the C-terminally truncated DOR was unchanged in CHO-T, but increased more slowly. Conclusion: DOR endocytosis is a vesicular and agonist-dependent process, but Receptors do not need signal transduction. The C-terminal end is unrelated to the membrane anchoring of the receptor but may affect the initiation of endocytosis of the receptor.