论文部分内容阅读
试用任何一个可能影响免疫状况的新药时,作为一个重要参数必须测定NK(自然杀伤)细胞的功能。但各试验室的测定结果常不能相比,而且同一个体在连续数周或数月测定时也有差异,因而使结果难于分析。本文介绍一种NK功能测定的标化方法可以克服这个缺点。材料和方法 1.淋巴细胞悬液的制备将肝素抗凝血中加入灭菌碳基铁(100mg/10ml血),37℃培育30分(不时摇动),反复通过2.5kg磁铁后取5ml加于3ml菲
When trying out any new drug that may affect the immune status, the function of NK (Natural Killer) cells must be determined as an important parameter. However, the results of each laboratory often can not be compared, and the same individual in a few weeks or months of continuous determination when there are differences, making the results difficult to analyze. This article describes a standardized method for determining NK function that overcomes this disadvantage. Materials and methods 1. Preparation of lymphocyte suspension Heparin anticoagulant added sterilized carbon-based iron (100mg / 10ml blood), incubated at 37 ℃ for 30 minutes (shaking from time to time), repeatedly through 2.5kg magnet 5ml added to 3ml Philippines