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慢性粒细胞白血病(CML)是一种克隆性血癌,其粒细胞和红细胞同源于造血干细胞。目前对CML患者粒细胞的质膜异常已有一些认识,而对其红细胞质膜却未见研究报告。本文报道CML患者红细胞质膜光谱异常的研究结果。经临床和血液学确诊的CML患者(年龄30～50岁),取静脉血置肝素抗凝的玻璃试管中,以Ficoll-Hypaque梯度分离红细胞,经2次离心分离后,混杂的白细胞和未成熟细胞<0.2%。CML患者和正常人的红细胞膜以SDS-聚丙烯凝胶电泳分析其蛋白质成分,并用凝胶光密度计于530nm处扫描观察红细胞的
Chronic myelogenous leukemia (CML) is a clonal blood cancer whose granulocytes and erythrocytes are homologous to hematopoietic stem cells. At present, there are some knowledge about the plasma membrane abnormalities of CML patients, but there is no research report on the plasma membrane of erythrocytes. This article reports the results of CML patients with erythrocyte plasma membrane abnormalities. Clinicians and hematology diagnosed CML patients (aged 30 to 50 years old), take venous blood to heparin anticoagulant glass tube, Ficoll-Hypaque gradient separation of red blood cells, after 2 centrifugation, mixed white blood cells and immature Cells <0.2%. The erythrocyte membranes of CML patients and normal individuals were analyzed for protein content by SDS-polyacrylamide gel electrophoresis and the erythrocytes were visualized by scanning with a gel densitometer at 530 nm